rDNA Sequencing and Preliminary Analysis of Microorganisms from Diseased Edible Fungus Using Non-culture Technology

The metagenomic DNA of disease tissue samples from four kinds of major edible fungus was extracted by CTAB method combined with DNA gel recovery kit. The genomie DNA was amplified by polymerase chain reaction using the universal primers of 16S rDNA and 18S rDNA, and then mone, elonal sequenced after...

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Published inPlant Diseases and Pests(植物病虫害研究：英文版) Vol. 5; no. 1; pp. 13 - 16
Main Authors Qiang, Yao, Zhiyuan, Gong, Neng, Gao, Jiandong, Han, Luzhang, Wan, Pengfei, Ren, Xiao, Liu, Chunyan, Huang, Haixia, Ren
Format Journal Article
LanguageEnglish
Published Cranston Wu Chu (USA-China) Science and Culture Media Corporation 01.02.2014
Subjects
rDNA序列
培养
基因组DNA
微生物技术
细菌性疾病
聚合酶链反应
通用引物
食用菌
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Summary:The metagenomic DNA of disease tissue samples from four kinds of major edible fungus was extracted by CTAB method combined with DNA gel recovery kit. The genomie DNA was amplified by polymerase chain reaction using the universal primers of 16S rDNA and 18S rDNA, and then mone, elonal sequenced after ligated and transformed, rDNA sequences of 20 positive clones were selected randomly from each pair of primers for sequence alignment. The results showed that there were two bacterial diseases and two fungul diseases in the samples, respectively.
Bibliography:Non-culture; Edible fungus disease; rDNA sequence analysis
The metagenomic DNA of disease tissue samples from four kinds of major edible fungus was extracted by CTAB method combined with DNA gel recovery kit. The genomie DNA was amplified by polymerase chain reaction using the universal primers of 16S rDNA and 18S rDNA, and then mone, elonal sequenced after ligated and transformed, rDNA sequences of 20 positive clones were selected randomly from each pair of primers for sequence alignment. The results showed that there were two bacterial diseases and two fungul diseases in the samples, respectively.
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ISSN:2152-3932