The P seudomonas aeruginosa AmrZ C ‐terminal domain mediates tetramerization and is required for its activator and repressor functions
Summary P seudomonas aeruginosa is an important bacterial opportunistic pathogen, presenting a significant threat towards individuals with underlying diseases such as cystic fibrosis. The transcription factor AmrZ regulates expression of multiple P . aeruginosa virulence factors. AmrZ belongs to the...
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Published in | Environmental microbiology reports Vol. 8; no. 1; pp. 85 - 90 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
01.02.2016
|
Online Access | Get full text |
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Summary: | Summary
P
seudomonas aeruginosa
is an important bacterial opportunistic pathogen, presenting a significant threat towards individuals with underlying diseases such as cystic fibrosis. The transcription factor
AmrZ
regulates expression of multiple
P
. aeruginosa
virulence factors.
AmrZ
belongs to the ribbon–helix–helix protein superfamily, in which many members function as dimers, yet others form higher order oligomers. In this study, four independent approaches were undertaken and demonstrated that the primary
AmrZ
form in solution is tetrameric. Deletion of the
AmrZ C
‐terminal domain leads to loss of tetramerization and reduced
DNA
binding to both activated and repressed target promoters. Additionally, the
C
‐terminal domain is essential for efficient
AmrZ
‐mediated activation and repression of its targets. |
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ISSN: | 1758-2229 1758-2229 |
DOI: | 10.1111/1758-2229.12354 |