KCNQ 1 OT 1, HIF 1A‐ AS 2 and APOA 1‐ AS are promising novel biomarkers for diagnosis of coronary artery disease

• Published in: Clinical and experimental pharmacology & physiology Vol. 46; no. 7; pp. 635 - 642
• Main Authors: Zhang, Yuan, Zhang, Lei, Wang, Yu, Ding, Han, Xue, Sheng, Yu, Hua, Hu, Longgang, Qi, Hongzhao, Wang, Yin, Zhu, Wenjie, Liu, Dacheng, Li, Peifeng
• Format: Journal Article
• Language: English
• Published: 01.07.2019
• ISSN: 0305-1870; 1440-1681
• DOI: 10.1111/1440-1681.13094

Abstract This study aimed to evaluate the potential of long noncoding RNA s (lnc RNA s) as biomarkers for coronary artery disease ( CAD ). We measured the levels of three atherosclerosis‐ or cardiac‐related lnc RNA s in peripheral blood monocyte cells ( PBMC s) from 20 CAD patients and 20 non‐ CAD control participants using real‐time reverse transcription–polymerase chain reaction (real‐time RT – PCR ) methods. We found that the levels of lnc RNA KCNQ 1 opposite strand/antisense transcript 1 ( KCNQ 1 OT 1), hypoxia‐inducible factor 1 alpha‐antisense RNA 2 ( HIF 1A‐ AS 2) and apolipoprotein A‐1 antisense RNA ( APOA 1‐ AS ) were significantly increased in CAD patients ( KCNQ 1 OT 1 increased by 2.38‐fold, P  = 0.00042; HIF 1A‐ AS 2 increased by 2.00‐fold, P  = 0.0001; APOA 1‐ AS increased by 4.52‐fold, P  = 0.000048). The area under the ROC curve was 0.865 for KCNQ 1 OT 1, 0.852 for HIF 1A‐ AS 2, and 0.967 for APOA 1‐ AS . Furthermore, the combination of lnc RNA s resulted in a much higher AUC value of 0.990 for the prediction of CAD . Spearman's correlation analysis showed that APOA 1‐ AS was positively correlated with NT ‐pro BNP , CKMB , MYO and HsTnT, whereas HIF 1A‐ AS 2 was correlated with NT ‐pro BNP and HsTnT. Hence, the elevation of KCNQ 1 OT 1, HIF 1A‐ AS 2 and APOA 1‐ AS predicts CAD and these molecules may be considered as novel biomarkers of CAD .