Cryo‐ EM structure of the Min CD copolymeric filament from Pseudomonas aeruginosa at 3.1 Å resolution
Positioning of the division site in many bacterial species relies on the Min CDE system, which prevents the cytokinetic Z‐ring from assembling anywhere but the mid‐cell, through an oscillatory diffusion‐reaction mechanism. MinD dimers bind to membranes and, via their partner MinC, inhibit the polyme...
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Published in | FEBS letters Vol. 593; no. 15; pp. 1915 - 1926 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
01.08.2019
|
Online Access | Get full text |
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Summary: | Positioning of the division site in many bacterial species relies on the Min
CDE
system, which prevents the cytokinetic Z‐ring from assembling anywhere but the mid‐cell, through an oscillatory diffusion‐reaction mechanism. MinD dimers bind to membranes and, via their partner MinC, inhibit the polymerization of cell division protein FtsZ into the Z‐ring. MinC and MinD form polymeric assemblies in solution and on cell membranes. Here, we report the high‐resolution cryo‐
EM
structure of the copolymeric filaments of
Pseudomonas aeruginosa
Min
CD
. The filaments consist of three protofilaments made of alternating MinC and MinD dimers. The Min
CD
protofilaments are almost completely straight and assemble as single protofilaments on lipid membranes, which we also visualized by cryo‐
EM
. |
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ISSN: | 0014-5793 1873-3468 |
DOI: | 10.1002/1873-3468.13471 |