A High Performance Electrochemical Biosensing Platform for Glucose Detection and IgE Aptasensing Based on Fe 3 O 4 /Reduced Graphene Oxide Nanocomposite

Abstract A Fe 3 O 4 magnetic nanoparticles/reduced graphene oxide nanocomposite modified glassy carbon electrode (Fe 3 O 4 /r‐GO/GCE) was further expanded into the design of a high performance electrochemical biosensing platform. First, direct electrochemistry of glucose oxidase(GOx) immobilized ont...

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Bibliographic Details
Published inElectroanalysis (New York, N.Y.) Vol. 26; no. 1; pp. 129 - 138
Main Authors Teymourian, Hazhir, Salimi, Abdollah, Firoozi, Somayeh
Format Journal Article
LanguageEnglish
Published 01.01.2014
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Summary:Abstract A Fe 3 O 4 magnetic nanoparticles/reduced graphene oxide nanocomposite modified glassy carbon electrode (Fe 3 O 4 /r‐GO/GCE) was further expanded into the design of a high performance electrochemical biosensing platform. First, direct electrochemistry of glucose oxidase(GOx) immobilized onto Fe 3 O 4 /r‐GO/GCE and its biocatalytic activity toward glucose oxidation was investigated. Then, differential pulse voltammetry (DPV) was used to investigate the electrochemical oxidation behavior of four DNA free bases of guanine (G), adenine (A), thymine (T) and Cytosine (C) at Fe 3 O 4 /r‐GO/GCE. In addition, a label‐free electrochemical impedance spectroscopy (EIS) method of detection was developed for Immunoglobulin E (IgE) in which the binding of IgE‐specific aptamers immobilized on Fe 3 O 4 /r‐GO/GCE leads to impedance changes associated with IgE binding events.
ISSN:1040-0397
1521-4109
DOI:10.1002/elan.201300496