Preparation of Supermacroporous Composite Cryogel Embedded with SiO_2 Nanoparticles

• Published in: Chinese journal of chemical engineering Vol. 18; no. 4; pp. 667 - 671
• Main Author: 许潘萍 姚雨辰 沈绍传 贠军贤 姚克俭
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 2010
• Subjects: composite cryogel; protein chromatography; SiO 2 nanoparticle; SiO2纳米粒子; 丙烯酰胺单体; 二氧化硅纳米粒子; 亚甲基双丙烯酰胺; 嵌入式; 纳米SiO2; SiO 2 nanoparticle; protein chromatography; composite cryogel
• ISSN: 1004-9541; 2210-321X
• DOI: 10.1016/S1004-9541(10)60273-X

Supermacroporous composite cryogels embedded with SiO2 nanoparticles were prepared by radical cryogenic copolymerization of the reactive monomer mixture of acrylamide（AAm） and N,N-methylene-bis-acrylamide（MBAAm） containing SiO2 nanoparticles（mass ratios of nanoparticles to the monomer AAm from 0.01 to 0.08） under the freezing-temperature variation condition in glass columns.The properties of these composite cryogels were measured.The height equivalent to theoretical plate（HETP） of the cryogel beds at different liquid flow rates was determined by residence time distribution（RTD） using tracer pulse-response method.The composite cryogel matrix embedded with the mass fraction of SiO2 nanoparticles of 0.02 presented the best properties and was employed in the following graft polymerization.Chromatographic process of lysozyme in the composite cryogel grafted with 2-acrylamido-2-methyl-1-propanesulfonic acid（AMPSA） was carried out to evaluate the protein breakthrough and elution characteristics.The chromatography can be carried out at relatively high superficial velocity,i.e.,15 cm·min-1,indicating the satisfactory mechanical strength due to the embedded nanoparticles.