Zooming in on a quantitative trait for tomato yield using interspecific introgressions

• Published in: Science (American Association for the Advancement of Science) Vol. 305; no. 5691; pp. 1786 - 1789
• Main Authors: Fridman, E, Carrari, F, Liu, Y.S, Fernie, A.R, Zamir, D
• Format: Journal Article
• Language: English
• Published: Washington, DC American Association for the Advancement of Science 17.09.2004; The American Association for the Advancement of Science
• Subjects: agronomic traits; Agronomy. Soil science and plant productions; Alleles; Amino Acid Sequence; amino acid sequences; Amino acids; Aspartic Acid; beta-fructofuranosidase; beta-Fructofuranosidase - chemistry; beta-Fructofuranosidase - genetics; beta-Fructofuranosidase - metabolism; Biodiversity; Biological and medical sciences; Biological diversity; brix; Brix9-2-5 locus; Catalytic Domain; Chromosomes; Crop science; Crosses, Genetic; Environmental Influences; enzyme activity; Enzyme kinetics; Flowers - enzymology; Flowers - genetics; food processing quality; Fruit - enzymology; Fruit - genetics; Fundamental and applied biological sciences. Psychology; Gene Expression; Gene Expression Regulation, Plant; Genes, Plant; Genetic aspects; Genetic Complementation Test; Genetics; Genetics and breeding of economic plants; Genotype & phenotype; Interspecific and intergeneric hybridization, introgressions; interspecific hybridization; introgression; introgression lines; lines; Lycopersicon esculentum; Lycopersicon esculentum - enzymology; Lycopersicon esculentum - genetics; Lycopersicon esculentum - growth & development; Molecular Sequence Data; Multifactorial traits; Ovaries; Phenotype; Phenotypes; Phenotypic traits; plant breeding; Plant breeding: fundamental aspects and methodology; Plants; Polymorphism, Single Nucleotide; Quantitative genetics; Quantitative Trait Loci; Quantitative Trait, Heritable; Quantitative traits; Solanum; Solanum - enzymology; Solanum - genetics; Solanum lycopersicum; Solanum lycopersicum var. lycopersicum; Solanum pennellii; Solanum pennellii var. pennellii; Sucrose - metabolism; sugar content; Sugars; Tomatoes; Transcription, Genetic; Transformation, Genetic; wild relatives; Yeasts; United States; Germany; Enzyme; Quantitative character; β-Fructofuranosidase; Introgression; Quantitative trait loci; Vegetable crop; Dicotyledones; Glycosidases; Angiospermae; Interspecific hybridization; Lycopersicon esculentum; Soluble sugar; Phenotype variation; Hydrolases; Wild plant; Carbohydrate; Spermatophyta; Yield; Solanaceae; O-Glycosidases
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1101666

To explore natural biodiversity we developed and examined introgression lines (ILs) containing chromosome segments of wild species (Solanum pennellii) in the background of the cultivated tomato (S. lycopersicum). We identified Brix9-2-5, which is a S. pennellii quantitative trait locus (QTL) that increases sugar yield of tomatoes and was mapped within a flower- and fruit-specific invertase (LIN5). QTL analysis representing five different tomato species delimited the functional polymorphism of Brix9-2-5 to an amino acid near the catalytic site of the invertase crystal, affecting enzyme kinetics and fruit sink strength. These results underline the power of diverse ILs for high-resolution perspectives on complex phenotypes.