Effects of Ion Irradiation on Seedlings Growth Monitored by Ultraweak Delayed Luminescence

• Published in: PloS one Vol. 11; no. 12; p. e0167998
• Main Authors: Grasso, Rosaria, Abe, Tomoko, Cirrone, Giuseppe A P, Cuttone, Giacomo, Gulino, Marisa, Musumeci, Francesco, Romano, Francesco, Ryuto, Hiromichi, Scordino, Agata
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 09.12.2016; Public Library of Science (PLoS)
• Subjects: Analysis; Astronomy; Biochemical analysis; Biodiversity; Biological effects; Biological properties; Biological samples; Biology and Life Sciences; Cancer therapies; Damage assessment; Dose-Response Relationship, Radiation; Elongation; Germination - radiation effects; Growth; Health aspects; Ion beams; Ion irradiation; Ionizing radiation; Irradiation; Luminescence; Mutation; Physical Sciences; Physics; Plant Development; Radiation; Radiation damage; Radiation dosage; Radiation, Ionizing; Research and Analysis Methods; Seedlings; Seeds; Seeds - radiation effects; Therapeutic applications; Tissues; Vegetables; Vigna radiata; Italy; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0167998

The optical technique based on the measurement of delayed luminescence emitted from the biological samples has demonstrated its ability to provide valid and predictive information on the functional status of various biological systems. We want to extend this technique to study the effect of ionizing radiation on biological systems. In particular we are interested in the action of ion beams, used for therapeutic purposes or to increase the biological diversity. In general, the assessment of the damage that radiation produces both in the target objects and in the surrounding tissues, requires considerable time because is based on biochemical analysis or on the examination of the evolution of the irradiated systems. The delayed luminescence technique could help to simplify this investigation. We have so started our studies performing irradiations of some relatively simple vegetable models. In this paper we report results obtained from mung bean (Vigna radiata) seeds submitted to a 12C ion beam at the energy of 62 MeV/nucleon. The dry seeds were irradiated at doses from 50 to 7000 Gy. The photoinduced delayed luminescence of each seed before and after ion irradiation was measured. The growth of seedlings after irradiation was compared with that of untreated seeds. A growth reduction on increasing the dose was registered. The results show strong correlations between the ion irradiation dose, seeds growth and delayed luminescence intensity. In particular, the delayed luminescence intensity is correlated by a logistic function to the seedlings elongation and, after performing a suitable measurement campaign based on blind tests, it could become a tool able to predict the growth of seeds after ion irradiation. Moreover these results demonstrate that measurements of delayed luminescence could be used as a fast and non-invasive technique to check the effects of ion beams on relatively simple biological systems.