CBOL protist working group: barcoding eukaryotic richness beyond the animal, plant, and fungal kingdoms

• Published in: PLoS biology Vol. 10; no. 11; p. e1001419
• Main Authors: Pawlowski, Jan, Audic, Stéphane, Adl, Sina, Bass, David, Belbahri, Lassaâd, Berney, Cédric, Bowser, Samuel S, Cepicka, Ivan, Decelle, Johan, Dunthorn, Micah, Fiore-Donno, Anna Maria, Gile, Gillian H, Holzmann, Maria, Jahn, Regine, Jirků, Miloslav, Keeling, Patrick J, Kostka, Martin, Kudryavtsev, Alexander, Lara, Enrique, Lukeš, Julius, Mann, David G, Mitchell, Edward A D, Nitsche, Frank, Romeralo, Maria, Saunders, Gary W, Simpson, Alastair G B, Smirnov, Alexey V, Spouge, John L, Stern, Rowena F, Stoeck, Thorsten, Zimmermann, Jonas, Schindel, David, de Vargas, Colomban
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.11.2012; Public Library of Science (PLoS)
• Subjects: Animals; Biodiversity; Biology; Cloning; DNA Barcoding, Taxonomic - methods; Ecosystem; Environmental Sciences; Eukaryota - classification; Eukaryota - genetics; Eukaryotic Cells - cytology; Fungi; Genetic Markers; Genetic Variation; Internet; Library collections; Life Sciences; Microbiology; Phylogeny; Plants - genetics; Ribosomes - genetics; RNA, Ribosomal, 18S - analysis; RNA, Ribosomal, 18S - genetics; Software; Species Specificity; Taxonomy; Eukaryotic Cells; Species Specificity; Ribosomes; Genetic Markers; Phylogeny; Plants; DNA Barcoding, Taxonomic; Genetic Variation; Animals; Eukaryota; Ecosystem; Software; Internet; RNA, Ribosomal, 18S
• ISSN: 1545-7885; 1544-9173; 1545-7885
• DOI: 10.1371/journal.pbio.1001419

Collection details including locality, date, and (as far as possible) habitat characteristics must also be provided, accompanied in parasitic and symbiotic taxa by an accurately identified host voucher or its DNA/tissue sample wherever this is available. [...]the extracted DNA must be deposited in a recognized DNA bank or museum collection and cited with a unique identifier to allow checks and further genetic analyses. [...]the large majority of protists are currently uncultivable by known means or not available in culture collections, and genetic data only exist for a very small fraction of described species. [...]it is imperative to establish standard barcoding protocols for future protist barcoding projects that will substantially increase the number of collected, described, but uncultivable protists.