用于浮游菌研究的微环境实验箱系统的研制
浮游菌是衡量环境洁净度的一项重要指标,然而洁净作业环境与受控实验环境的特殊性往往导致浮游菌的研究比较受限。本文设计并实现了一种微环境实验箱系统,便于在常规实验条件下进行浮游菌研究。Fluent数值模拟表明,浮游菌在实验箱内中上部具备较好的分布状态,并呈现出自下而上的浓度增长分布现象。根据模拟结果,在实验箱内选择不同空间位置的5个测量点进行粒子计数浓度验证实验。实验结果表明金黄色葡萄球菌发菌5 min后各测量点平均粒子浓度均达到了107个/m^3数量级,各测量点粒子分布较为一致,且上部浓度略高于中部浓度,中部浓度略高于底部浓度。验证实验结果与数值模拟结果相符,证明该系统能够较好地用于浮游菌研究。...
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| Published in | Sheng wu yi xue gong cheng xue za zhi Vol. 34; no. 5; pp. 778 - 783 |
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| Main Author | |
| Format | Journal Article |
| Language | Chinese English |
| Published |
中国四川
四川大学华西医院
01.10.2017
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| Subjects | |
| Online Access | Get full text |
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| Summary: | 浮游菌是衡量环境洁净度的一项重要指标,然而洁净作业环境与受控实验环境的特殊性往往导致浮游菌的研究比较受限。本文设计并实现了一种微环境实验箱系统,便于在常规实验条件下进行浮游菌研究。Fluent数值模拟表明,浮游菌在实验箱内中上部具备较好的分布状态,并呈现出自下而上的浓度增长分布现象。根据模拟结果,在实验箱内选择不同空间位置的5个测量点进行粒子计数浓度验证实验。实验结果表明金黄色葡萄球菌发菌5 min后各测量点平均粒子浓度均达到了107个/m^3数量级,各测量点粒子分布较为一致,且上部浓度略高于中部浓度,中部浓度略高于底部浓度。验证实验结果与数值模拟结果相符,证明该系统能够较好地用于浮游菌研究。 |
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| Bibliography: | airborne microbe; microenvironment; test chamber 51-1258/R One of the most important environmental cleanliness indicators is airborne microbe. However, the particularity of clean operating environment and controlled experimental environment often leads to the limitation of the airborne microbe research. This paper designed and implemented a microenvironment test chamber for airborne microbe research in normal test conditions. Numerical simulation by Fluent showed that airborne microbes were evenly dispersed in the upper part of test chamber, and had a bottom-up concentration growth distribution. According to the simulation results, the verification experiment was carried out by selecting 5 sampling points in different space positions in the test chamber. Experimental results showed that average particle concentrations of all sampling points reached 107 counts/m^3 after 5 minutes＇ distributing of Staphylococcus aureus, and all sampling points showed the accordant mapping of concentration distribution. The concen |
| ISSN: | 1001-5515 |
| DOI: | 10.7507/1001-5515.201609005 |