Human bone marrow harbors cells with neural crest-associated characteristics like human adipose and dermis tissues

• Published in: PloS one Vol. 12; no. 7; p. e0177962
• Main Authors: Coste, Cécile, Neirinckx, Virginie, Sharma, Anil, Agirman, Gulistan, Rogister, Bernard, Foguenne, Jacques, Lallemend, François, Gothot, André, Wislet, Sabine
• Format: Journal Article Web Resource
• Language: English
• Published: United States Public Library of Science 06.07.2017; Public Library of Science (PLoS)
• Subjects: Adipose tissue; Adipose Tissue - cytology; Adipose Tissue - metabolism; Adipose Tissue/cytology/metabolism; Adult; Animals; Biology and Life Sciences; Biomarkers - metabolism; Bone marrow; Bone Marrow Cells - cytology; Bone Marrow Cells - metabolism; Bone Marrow Cells/cytology/metabolism; Brn-3 protein; Cell adhesion & migration; Cell Differentiation; Cell- och molekylärbiologi; Chick Embryo; Comparative analysis; Dental pulp; Dermis; Dermis - cytology; Dermis - metabolism; Dermis/cytology/metabolism; Embryos; Ethics; Female; Gene Expression; Hair; Hematology; Human health sciences; Human performance; Human tissues; Humans; Hématologie; Immunology; Laboratories; Medicin och hälsovetenskap; Medicine and Health Sciences; Medicinsk bioteknologi; Medicinsk bioteknologi (inriktn. mot cellbiologi (inkl. stamcellsbiologi), molekylärbiologi, mikrobiologi, biokemi eller biofarmaci); Medicinska och farmaceutiska grundvetenskaper; Melanocytes; Melanocytes - cytology; Melanocytes - metabolism; Melanocytes/cytology/metabolism; Mesenchymal Stem Cell Transplantation; Mesenchymal stem cells; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - metabolism; Mesenchymal Stromal Cells/cytology/metabolism; Microinjections; Msi1 protein; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Nerve Tissue Proteins/genetics/metabolism; Nervous system; Nestin; Nestin - genetics; Nestin - metabolism; Nestin/genetics/metabolism; Neural crest; Neural Crest - cytology; Neural Crest - growth & development; Neural Crest - metabolism; Neural Crest/cytology/growth & development/metabolism; Neural plasticity; Neural Stem Cells - cytology; Neural Stem Cells - metabolism; Neural Stem Cells/cytology/metabolism; Neurons; Neurons - cytology; Neurons - metabolism; Neurons/cytology/metabolism; Neurosciences; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Nuclear Proteins/genetics/metabolism; People and Places; Receptors, Nerve Growth Factor - genetics; Receptors, Nerve Growth Factor - metabolism; Receptors, Nerve Growth Factor/genetics/metabolism; Regenerative medicine; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; RNA-Binding Proteins/genetics/metabolism; Schwann cells; Schwann Cells - cytology; Schwann Cells - metabolism; Schwann Cells/cytology/metabolism; Sciences de la santé humaine; Skin; Snail Family Transcription Factors - genetics; Snail Family Transcription Factors - metabolism; Snail Family Transcription Factors/genetics/metabolism; Sox9 protein; SOX9 Transcription Factor - genetics; SOX9 Transcription Factor - metabolism; SOX9 Transcription Factor/genetics/metabolism; Stem cells; Stromal cells; Surgery; Tissue engineering; Transcription Factor Brn-3A - genetics; Transcription Factor Brn-3A - metabolism; Transcription Factor Brn-3A/genetics/metabolism; Twist-Related Protein 1 - genetics; Twist-Related Protein 1 - metabolism; Twist-Related Protein 1/genetics/metabolism; Belgium
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0177962

Adult neural crest stem-derived cells (NCSC) are of extraordinary high plasticity and promising candidates for use in regenerative medicine. Several locations such as skin, adipose tissue, dental pulp or bone marrow have been described in rodent, as sources of NCSC. However, very little information is available concerning their correspondence in human tissues, and more precisely for human bone marrow. The main objective of this study was therefore to characterize NCSC from adult human bone marrow. In this purpose, we compared human bone marrow stromal cells to human adipose tissue and dermis, already described for containing NCSC. We performed comparative analyses in terms of gene and protein expression as well as functional characterizations. It appeared that human bone marrow, similarly to adipose tissue and dermis, contains NESTIN+ / SOX9+ / TWIST+ / SLUG+ / P75NTR+ / BRN3A+/ MSI1+/ SNAIL1+ cells and were able to differentiate into melanocytes, Schwann cells and neurons. Moreover, when injected into chicken embryos, all those cells were able to migrate and follow endogenous neural crest migration pathways. Altogether, the phenotypic characterization and migration abilities strongly suggest the presence of neural crest-derived cells in human adult bone marrow.