基于太赫兹时域光谱的转基因与非转基因棉花种子鉴别
太赫兹光谱是介于红外与微波间的电磁波谱,很多生物大分子在该波段具有指纹特性.为探索棉花种子的转基因机理和性状表达的太赫兹光谱特性,该文利用太赫兹时域光谱技术对3种转基因棉花种子和1种常规育种棉花种子进行了鉴别试验探索研究.试验首先研究了棉花种子压片制备方法,然后借助太赫兹时域光谱设备,采集了4种棉花种子在有效频率范围内的太赫兹光谱信息,进而对太赫兹光谱包含的特征信息进行了定性分析,并初步探讨了4种样品的太赫兹吸收特性.试验结果表明:4种棉花种子在太赫兹波段呈现不同的光谱响应特性,尤其是在其吸光系数光谱图中,3种转基因棉种在1.21、1.23和1.41 THz处,对太赫兹呈现明显的吸收,而非转基...
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Published in | 农业工程学报 Vol. 33; no. z1; pp. 288 - 292 |
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Main Author | |
Format | Journal Article |
Language | Chinese |
Published |
农业部农业信息技术重点实验室,北京 100097%石河子大学机械电气工程学院,石河子,832000%国家农业信息化工程技术研究中心,北京,100097
2017
国家农业信息化工程技术研究中心,北京 100097 石河子大学机械电气工程学院,石河子 832000%国家农业信息化工程技术研究中心,北京 100097 |
Subjects | |
Online Access | Get full text |
ISSN | 1002-6819 |
DOI | 10.11975/j.issn.1002-6819.2017.z1.043 |
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Summary: | 太赫兹光谱是介于红外与微波间的电磁波谱,很多生物大分子在该波段具有指纹特性.为探索棉花种子的转基因机理和性状表达的太赫兹光谱特性,该文利用太赫兹时域光谱技术对3种转基因棉花种子和1种常规育种棉花种子进行了鉴别试验探索研究.试验首先研究了棉花种子压片制备方法,然后借助太赫兹时域光谱设备,采集了4种棉花种子在有效频率范围内的太赫兹光谱信息,进而对太赫兹光谱包含的特征信息进行了定性分析,并初步探讨了4种样品的太赫兹吸收特性.试验结果表明:4种棉花种子在太赫兹波段呈现不同的光谱响应特性,尤其是在其吸光系数光谱图中,3种转基因棉种在1.21、1.23和1.41 THz处,对太赫兹呈现明显的吸收,而非转基因棉种却呈现微弱的吸收或不吸收;这些明显的差异表明太赫兹光谱可以作为鉴别转基因/非转基因棉花种子有效工具,该研究可为进一步揭示太赫兹光谱检测机理,发展转/非转基因物质快速检测方法提供参考. |
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Bibliography: | spectroscopy;cotton;seed;Terahertz;identification;transgenic;absorption coefficient 11-2047/S Terahertz radiation is the electromagnetic wave, which occupies a large portion between the infrared and microwave bands. Most biological macromolecules have fingerprint characteristics in this band. Firstly, the device for experiment i.e. the terahertz time-domain spectroscopy system (THz-TDS) was introduced. Then, in order to seek the terahertz spectrum characteristics of transgenic mechanism and the trait expression, 3 kinds of transgenic cotton seeds (Zhongmian No.838, Lumianyan No.28, and Guoshen No.7886) and a non-transgenic cotton seed (Xinluzao No.51) were studied in identifying experiments by terahertz time-domain spectroscopy. Because of the grease-like substance in the cotton seed, tablet mold was occlusive when compressing cotton seed. It made the tablet shaping difficult and the surface of the tablets rough and resulted in unperfect data. To solve the problem, when compressing tablet, a small amount of pol |
ISSN: | 1002-6819 |
DOI: | 10.11975/j.issn.1002-6819.2017.z1.043 |