Differential aging of growth plate cartilage underlies differences in bone length and thus helps determine skeletal proportions

• Published in: PLoS biology Vol. 16; no. 7; p. e2005263
• Main Authors: Lui, Julian C, Jee, Youn Hee, Garrison, Presley, Iben, James R, Yue, Shanna, Ad, Michal, Nguyen, Quang, Kikani, Bijal, Wakabayashi, Yoshiyuki, Baron, Jeffrey
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 23.07.2018; Public Library of Science (PLoS)
• Subjects: Aging; Bioinformatics; Biology; Biology and Life Sciences; Bone growth; Bone morphogenetic proteins; Bones; Cartilage; Cell proliferation; Childrens health; Consortia; Elongation; Gene expression; Genes; Genomes; Genomics; Growth factors; Growth plate; Growth rate; Hypertrophy; Insulin; Insulin-like growth factors; Labeling; Medicine and Health Sciences; Molecular modelling; Ontology; Paracrine signalling; Proteins; Research and analysis methods; Senescence; Short Reports; Software; Structure-function relationships; Tissue engineering; Wnt protein; England; United Kingdom > UK; United States > US; Maryland
• ISSN: 1545-7885; 1544-9173; 1545-7885
• DOI: 10.1371/journal.pbio.2005263

Bones at different anatomical locations vary dramatically in size. For example, human femurs are 20-fold longer than the phalanges in the fingers and toes. The mechanisms responsible for these size differences are poorly understood. Bone elongation occurs at the growth plates and advances rapidly in early life but then progressively slows due to a developmental program termed "growth plate senescence." This developmental program includes declines in cell proliferation and hypertrophy, depletion of cells in all growth plate zones, and extensive underlying changes in the expression of growth-regulating genes. Here, we show evidence that these functional, structural, and molecular senescent changes occur earlier in the growth plates of smaller bones (metacarpals, phalanges) than in the growth plates of larger bones (femurs, tibias) and that this differential aging contributes to the disparities in bone length. We also show evidence that the molecular mechanisms that underlie the differential aging between different bones involve modulation of critical paracrine regulatory pathways, including insulin-like growth factor (Igf), bone morphogenetic protein (Bmp), and Wingless and Int-1 (Wnt) signaling. Taken together, the findings reveal that the striking disparities in the lengths of different bones, which characterize normal mammalian skeletal proportions, is achieved in part by modulating the progression of growth plate senescence.