IN VITRO INVESTIGATION OF ANTIOXIDANT AND ANTI-UROLITHIATIC ACTIVITY OF HOMONOIA RETUSA ROOTS
Ayurvedic practitioners utilise Pashanabheda as an anti-urolithiatic. The root of Homonoia retusa (Euphorbiaceae), which grows in the Indian town of Tirupathi, was chosen for this study's screening for its antioxidant and anti-urolithiatic properties. The hydrogen peroxide assay (H2O2), ferric...
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Published in | International journal of research in ayurveda and pharmacy Vol. 15; no. 1; pp. 98 - 103 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
29.02.2024
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Online Access | Get full text |
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Summary: | Ayurvedic practitioners utilise Pashanabheda as an anti-urolithiatic. The root of Homonoia retusa (Euphorbiaceae), which grows in the Indian town of Tirupathi, was chosen for this study's screening for its antioxidant and anti-urolithiatic properties. The hydrogen peroxide assay (H2O2), ferric reducing antioxidant power (FRAP), and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) techniques were utilised to assess antioxidant activity. The anti-urolithiatic activity was evaluated by nucleation and aggregation assay by adding 0.01 M sodium oxalate solutions that caused the crystallisation of Calcium oxalate. By measuring turbidity in the presence or absence of extract at 620 nm using a spectrophotometer, the effect of extract (200, 400, 600, 800, and 1000 µg/ml) was investigated. According to the result, aqueous root extract can prevent crystal growth and aggregation more than methanol-based root extract. |
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ISSN: | 2229-3566 2229-3566 |
DOI: | 10.7897/2277-4343.15121 |