Structure and function of a transcriptional network activated by the MAPK Hog1

• Published in: Nature genetics Vol. 40; no. 11; pp. 1300 - 1306
• Main Authors: O'Shea, Erin K, Capaldi, Andrew P, Kaplan, Tommy, Liu, Ying, Habib, Naomi, Regev, Aviv, Friedman, Nir
• Format: Journal Article
• Language: English
• Published: New York Nature Publishing Group US 01.11.2008; Nature Publishing Group
• Subjects: Agriculture; Animal Genetics and Genomics; Binding Sites; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Cancer Research; Cellular signal transduction; Chromatin Immunoprecipitation; Enzyme Activation; Fundamental and applied biological sciences. Psychology; Gene expression; Gene Expression Regulation, Fungal; Gene Function; Gene Regulatory Networks; Genetic aspects; Genetic regulation; Genetics of eukaryotes. Biological and molecular evolution; Human Genetics; Kinases; Life sciences; Matrix; Mitogen-Activated Protein Kinases - chemistry; Mitogen-Activated Protein Kinases - metabolism; Models, Biological; Molecular and cellular biology; Molecular genetics; Mutation - genetics; Normal distribution; Osmotic Pressure; Physiological aspects; Protein kinases; Saccharomyces cerevisiae; Saccharomyces cerevisiae - enzymology; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae Proteins - chemistry; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Signal Transduction; Transcription. Transcription factor. Splicing. Rna processing; Yeasts; United States; Mitogen-activated protein kinase; Transcription; Enzyme; Transferases
• ISSN: 1061-4036; 1546-1718
• DOI: 10.1038/ng.235

Erin O'Shea and colleagues present a quantitative model of the Hog1 MAPK-dependent osmotic stress response in budding yeast derived from gene expression analyses in single- and multiple-mutant strains. The network reveals interactions involved in signal integration and processing and could serve as model for investigations into other gene regulatory networks. Cells regulate gene expression using a complex network of signaling pathways, transcription factors and promoters. To gain insight into the structure and function of these networks, we analyzed gene expression in single- and multiple-mutant strains to build a quantitative model of the Hog1 MAPK-dependent osmotic stress response in budding yeast. Our model reveals that the Hog1 and general stress (Msn2/4) pathways interact, at both the signaling and promoter level, to integrate information and create a context-dependent response. This study lays out a path to identifying and characterizing the role of signal integration and processing in other gene regulatory networks.