The functions of myosin II and myosin V homologs in tip growth and septation in Aspergillus nidulans

• Published in: PloS one Vol. 7; no. 2; p. e31218
• Main Authors: Taheri-Talesh, Naimeh, Xiong, Yi, Oakley, Berl R
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 16.02.2012; Public Library of Science (PLoS)
• Subjects: Amino acids; Aspergillus fumigatus; Aspergillus nidulans; Aspergillus nidulans - growth & development; Aspergillus nidulans - ultrastructure; Biology; Chitin; Coalescing; Contractility; Cytoplasm; Cytoskeleton; DNA damage; Fungal Proteins; Fungi; Genes; Genome, Fungal; Genomes; Genomics; Growth; Homology; Medical importance; Muscle proteins; Myosin; Myosin Type II - physiology; Myosin Type V - physiology; Proteins; Secretion; Septation; Septum; SNAP receptors; Strings; Time-Lapse Imaging; Vesicles; Yeast; Ohio; Kansas; California; United States > US
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0031218

Because of the industrial and medical importance of members of the fungal genus Aspergillus, there is considerable interest in the functions of cytoskeletal components in growth and secretion in these organisms. We have analyzed the genome of Aspergillus nidulans and found that there are two previously unstudied myosin genes, a myosin II homolog, myoB (product = MyoB) and a myosin V homolog, myoE (product = MyoE). Deletions of either cause significant growth defects. MyoB localizes in strings that coalesce into contractile rings at forming septa. It is critical for septation and normal deposition of chitin but not for hyphal extension. MyoE localizes to the Spitzenkörper and to moving puncta in the cytoplasm. Time-lapse imaging of SynA, a v-SNARE, reveals that in myoE deletion strains vesicles no longer localize to the Spitzenkörper. Tip morphology is slightly abnormal and branching occurs more frequently than in controls. Tip extension is slower than in controls, but because hyphal diameter is greater, growth (increase in volume/time) is only slightly reduced. Concentration of vesicles into the Spitzenkörper before incorporation into the plasma membrane is, thus, not required for hyphal growth but facilitates faster tip extension and a more normal hyphal shape.