Comparative analysis of bacterial communities in a potato field as determined by pyrosequencing

• Published in: PloS one Vol. 6; no. 8; p. e23321
• Main Authors: Inceoğlu, Özgül, Al-Soud, Waleed Abu, Salles, Joana Falcão, Semenov, Alexander V, van Elsas, Jan Dirk
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19.08.2011; Public Library of Science (PLoS)
• Subjects: Abundance; Agriculture; Bacteria; Bacteria - classification; Bacteria - genetics; Biology; Cluster Analysis; Communities; Comparative analysis; Cultivars; Deoxyribonucleic acid; DNA; DNA sequencing; Dominant species; Ecological conditions; Ecology; Flowering; Flowers - physiology; Growth stage; Laws, regulations and rules; Life sciences; Methods; Microorganisms; Plant communities; Polymerase chain reaction; Potatoes; Principal Component Analysis; Rare species; Rhizosphere; RNA, Ribosomal, 16S - genetics; Seasons; Senescence; Sequence Analysis, DNA - methods; Soil analysis; Soil Microbiology; Soil microorganisms; Soils; Solanum tuberosum - growth & development; Solanum tuberosum - microbiology; Starch; Temperature; Tubers; Netherlands
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0023321

Plants selectively attract particular soil microorganisms, in particular consumers of root-excreted compounds. It is unclear to what extent cultivar type and/or growth stage affect this process. DNA-based pyrosequencing was used to characterize the structure of bacterial communities in a field cropped with potato. The rhizospheres of six cultivars denoted Aveka, Aventra, Karnico, Modena, Premiere and Desiree, at three growth stages (young, flowering and senescence) were examined, in addition to corresponding bulk soils. Around 350,000 sequences were obtained (5,700 to 38,000 per sample). Across all samples, rank abundance distributions best fitted the power law model, which indicates a community composed of a few highly dominant species next to numerous rare species. Grouping of the sequences showed that members of the Actinobacteria, Alphaproteobacteria, next to as-yet-unclassified bacteria, dominated. Other groups that were consistently found, albeit at lower abundance, were Beta-, Gamma- and Deltaproteobacteria and Acidobacteria. Principal components analyses revealed that rhizosphere samples were significantly different from corresponding bulk soil in each growth stage. Furthermore, cultivar effects were found in the young plant stage, whereas these became insignificant in the flowering and senescence stages. Besides, an effect of time of season was observed for both rhizosphere and bulk soils. The analyzed rhizosphere samples of the potato cultivars were grouped into two groups, in accordance with the allocation of carbon to starch in their tubers, i.e. Aveka, Aventra and Karnico (high) versus Premiere and Desiree (low) and thus replicates per group were established. Across all potato cultivars, the young plant stages revealed cultivar-dependent bacterial community structures, which disappeared in the flowering and senescence stages. Furthermore, Pseudomonas, Beta-, Alpha- and Deltaproteobacteria flourished under different ecological conditions than the Acidobacteria.