The delay in the development of experimental colitis from isomaltosyloligosaccharides in rats is dependent on the degree of polymerization

• Published in: PloS one Vol. 7; no. 11; p. e50658
• Main Authors: Iwaya, Hitoshi, Lee, Jae-Sung, Yamagishi, Shinya, Shinoki, Aki, Lang, Weeranuch, Thawornkuno, Charin, Kang, Hee-Kwon, Kumagai, Yuya, Suzuki, Shiho, Kitamura, Shinichi, Hara, Hiroshi, Okuyama, Masayuki, Mori, Haruhide, Kimura, Atsuo, Ishizuka, Satoshi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 29.11.2012; Public Library of Science (PLoS)
• Subjects: Animals; Biology; Bleeding; Body weight; Body weight loss; Butyric acid; Carbohydrates; Cecum; Colitis; Colitis - chemically induced; Colon; Cytokines; Degree of polymerization; Dextran; Dextran sulfate; Dextran Sulfate - toxicity; Dextrans; Dextrans - adverse effects; Dextrans - chemistry; Diet; Digestibility; Environmental science; Fatty acids; Glucagon; Glucagon-like peptide 2; Inflammatory bowel disease; Laboratory animals; Leukocytes (neutrophilic); Male; Medicine; Mucosa; Nutrition research; Oligosaccharides - adverse effects; Oligosaccharides - chemistry; Peptides; Peripheral blood; Peroxidase; Physiological aspects; Physiological effects; Physiology; Polymerization; Rats; Rats, Wistar; Rectum; Rodents; Saccharides; Small intestine; Sodium; Structure-Activity Relationship; Sulfate; Sulfates; Tumor necrosis factor-TNF; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0050658

Isomaltosyloligosaccharides (IMO) and dextran (Dex) are hardly digestible in the small intestine and thus influence the luminal environment and affect the maintenance of health. There is wide variation in the degree of polymerization (DP) in Dex and IMO (short-sized IMO, S-IMO; long-sized IMO, L-IMO), and the physiological influence of these compounds may be dependent on their DP. Five-week-old male Wistar rats were given a semi-purified diet with or without 30 g/kg diet of the S-IMO (DP = 3.3), L-IMO (DP = 8.4), or Dex (DP = 1230) for two weeks. Dextran sulfate sodium (DSS) was administered to the rats for one week to induce experimental colitis. We evaluated the clinical symptoms during the DSS treatment period by scoring the body weight loss, stool consistency, and rectal bleeding. The development of colitis induced by DSS was delayed in the rats fed S-IMO and Dex diets. The DSS treatment promoted an accumulation of neutrophils in the colonic mucosa in the rats fed the control, S-IMO, and L-IMO diets, as assessed by a measurement of myeloperoxidase (MPO) activity. In contrast, no increase in MPO activity was observed in the Dex-diet-fed rats even with DSS treatment. Immune cell populations in peripheral blood were also modified by the DP of ingested saccharides. Dietary S-IMO increased the concentration of n-butyric acid in the cecal contents and the levels of glucagon-like peptide-2 in the colonic mucosa. Our study provided evidence that the physiological effects of α-glucosaccharides on colitis depend on their DP, linkage type, and digestibility.