Relationship between glass transition temperature, and desiccation and heat tolerance in Salmonella enterica

• Published in: PloS one Vol. 15; no. 5; p. e0233638
• Main Authors: Lee, Kyeongmin, Shoda, Masaki, Kawai, Kiyoshi, Koseki, Shigenobu
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 29.05.2020; Public Library of Science (PLoS)
• Subjects: Bacteria; Bacterial cell walls; Biology and Life Sciences; Deactivation; Desiccation; Environmental changes; Epidemics; Food; Glass transition temperature; Health aspects; Heat; Heat inactivation; Heat tolerance; Heat tolerance (Biology); Inactivation; Medicine and Health Sciences; Microbiological research; Organisms; Physical Sciences; Physiological aspects; Potassium; Research and Analysis Methods; Rheological properties; Rheology; Salmonella; Salmonella enterica; Softening; Survival; Temperature tolerance; Thermal stress; Transition state (Chemistry); Transition temperatures; Water activity; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0233638

Pathogenic bacteria such as Salmonella enterica exhibit high desiccation tolerance, enabling long-term survival in low water activity (aw) environments. Although there are many reports on the effects of low aw on bacterial survival, the mechanism by which bacteria acquire desiccation tolerance and resistance to heat inactivation in low-aw foods remains unclear. We focused on the glass transition phenomenon, as bacteria may acquire environmental tolerance by state change due to glass transition. In this study, we determined the glass transition temperature (Tg) in S. enterica serovars under different aw conditions using thermal rheological analysis (TRA). The softening behaviour associated with the state change of bacterial cells was confirmed by TRA, and Tg was determined from the softening behaviour. Tg increased as the aw decreased in all S. enterica serovars. For example, while the Tg of five S. enterica serovars was determined as 35.16°C to 57.46°C at 0.87 aw, the Tg of all the five serovars increased by 77.10°C to 83.30°C at 0.43 aw. Furthermore, to verify the thermal tolerance of bacterial cells, a thermal inactivation assay was conducted at 60°C for 10 min under each aw condition. A higher survival ratio was observed as aw decreased; this represented an increase in Tg for Salmonella strains. These results suggest that the glass transition phenomenon of bacterial cells would associate with environmental tolerance.