Study of Escherichia coli, EPEC, ETEC AND STEC, in water offered in schools in a municipality with low human development index in Maranhão State, Brazil
This study examined the EPEC, ETEC and STEC pathotypes in E. coli isolates from water supplied to schools in a municipality in Maranhão, Brazil with a low human development index. For this, 57 bacterial strains isolated from 19 water samples were used. All strains were confirmed as belonging to the...
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Published in | Revista ambiente & água Vol. 19; pp. 1 - 8 |
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Main Authors | , , , , , , , |
Format | Journal Article |
Language | English |
Published |
20.09.2024
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Online Access | Get full text |
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Summary: | This study examined the EPEC, ETEC and STEC pathotypes in E. coli isolates from water supplied to schools in a municipality in Maranhão, Brazil with a low human development index. For this, 57 bacterial strains isolated from 19 water samples were used. All strains were confirmed as belonging to the E. coli species, by Gram staining and phenotypic tests. From pure cultures of E. coli, DNA was extracted followed by characterization of the isolates by polymerase chain reaction (PCR). This is the first study carried out in the state of Maranhão on the research of the diarrheagenic strains EPEC, ETEC and STEC in water for human consumption offered in schools, with the detection of virulent genes characteristic of enterotoxigenic E. coli (26.31%; n = 15/57) and E. coli producing Shiga toxin (8.78%; n= 5/57). Of the strains identified, 8.78% (n= 5/57) corresponded to combinations of est + stx1 genes. It is concluded that the detection of the diarrheagenic strains ETEC and STEC in E. coli isolates from water samples for human consumption indicates that, in addition to the water being contaminated, it harbors strains with pathogenic potential to cause diarrheal infection in its users. Keywords: ETEC, school environment, STEC. |
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ISSN: | 1980-993X 1980-993X |
DOI: | 10.4136/ambi-agua.3002 |