Mesenchymal Stem Cell Therapy for the Treatment of Vocal Fold Scarring: A Systematic Review of Preclinical Studies

• Published in: PloS one Vol. 11; no. 9; p. e0162349
• Main Authors: Wingstrand, Vibe Lindeblad, Grønhøj Larsen, Christian, Jensen, David H, Bork, Kristian, Sebbesen, Lars, Balle, Jesper, Fischer-Nielsen, Anne, von Buchwald, Christian
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 15.09.2016; Public Library of Science (PLoS)
• Subjects: Animals; Audiology; Biology and Life Sciences; Biomechanics; Bone marrow; Cicatrix - therapy; Clinical trials; Control methods; Extracellular matrix; Humans; Injury prevention; Larynx; Mechanical properties; Medicine and Health Sciences; Mesenchymal stem cells; Mesenchymal Stem Cells - cytology; Mesenchyme; Mucosa; Otolaryngology; Otology; Physical Sciences; Proteins; Research and Analysis Methods; Scars; Scientific papers; Search engines; Skin & tissue grafts; Stem Cell Transplantation; Stem cells; Stiffness; Storage modulus; Studies; Surgery; Therapy; Viscoelasticity; Viscosity; Vocal Cords - pathology; Wound healing; Denmark
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0162349

Therapy with mesenchymal stem cells exhibits potential for the development of novel interventions for many diseases and injuries. The use of mesenchymal stem cells in regenerative therapy for vocal fold scarring exhibited promising results to reduce stiffness and enhance the biomechanical properties of injured vocal folds. This study evaluated the biomechanical effects of mesenchymal stem cell therapy for the treatment of vocal fold scarring. PubMed, Embase, the Cochrane Library and Google Scholar were searched. Controlled studies that assessed the biomechanical effects of mesenchymal stem cell therapy for the treatment of vocal fold scarring were included. Primary outcomes were viscoelastic properties and mucosal wave amplitude. Seven preclinical animal studies (n = 152 single vocal folds) were eligible for inclusion. Evaluation of viscoelastic parameters revealed a decreased dynamic viscosity (η') and elastic modulus (G'), i.e., decreased resistance and stiffness, in scarred vocal folds treated with mesenchymal stem cells compared to non-treated scarred vocal folds. Mucosal wave amplitude was increased in scarred vocal folds treated with mesenchymal stem cells vs. non-treated scarred vocal folds. The results from these studies suggest an increased regenerative effect of therapy with mesenchymal stem cells for scarred vocal folds and are encouraging for further clinical studies.