Life History Responses and Gene Expression Profiles of the Nematode Pristionchus pacificus Cultured on Cryptococcus Yeasts

• Published in: PloS one Vol. 11; no. 10; p. e0164881
• Main Authors: Sanghvi, Gaurav V, Baskaran, Praveen, Röseler, Waltraud, Sieriebriennikov, Bogdan, Rödelsperger, Christian, Sommer, Ralf J
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 14.10.2016; Public Library of Science (PLoS)
• Subjects: Analysis; Animal behavior; Animals; Bacteria; Beetles; Biology and Life Sciences; Caenorhabditis elegans; Caenorhabditis elegans - genetics; Caenorhabditis elegans - growth & development; Caenorhabditis elegans - metabolism; Cryptococcus; Cryptococcus - physiology; Cryptococcus albidus; Cryptococcus curvatus; Developmental biology; E coli; Ecosystems; Environmental changes; Environmental conditions; Escherichia coli; Evolution; Evolutionary biology; Feeders; Food; Food plants; Food sources; Fungal infections; Fungi; Gene expression; Genomes; Laboratories; Life Cycle Stages; Life history; Medicine and Health Sciences; Metazoa; Microscopy; Nematoda; Nematoda - genetics; Nematoda - growth & development; Nematoda - metabolism; Nematodes; Parasites; Physiology; Prey; Pristionchus; Pristionchus pacificus; Research and Analysis Methods; Roundworms; Strains (organisms); Studies; Transcriptome; Yeast; Yeasts
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0164881

Nematodes, the earth's most abundant metazoa are found in all ecosystems. In order to survive in diverse environments, they have evolved distinct feeding strategies and they can use different food sources. While some nematodes are specialists, including parasites of plants and animals, others such as Pristionchus pacificus are omnivorous feeders, which can live on a diet of bacteria, protozoans, fungi or yeast. In the wild, P. pacificus is often found in a necromenic association with beetles and is known to be able to feed on a variety of microbes as well as on nematode prey. However, in laboratory studies Escherichia coli OP50 has been used as standard food source, similar to investigations in Caenorhabditis elegans and it is unclear to what extent this biases the obtained results and how relevant findings are in real nature. To gain first insight into the variation in traits induced by a non-bacterial food source, we study Pristionchus-fungi interactions under laboratory conditions. After screening different yeast strains, we were able to maintain P. pacificus for at least 50-60 generations on Cryptococcus albidus and Cryptococcus curvatus. We describe life history traits of P. pacificus on both yeast strains, including developmental timing, survival and brood size. Despite a slight developmental delay and problems to digest yeast cells, which are both reflected at a transcriptomic level, all analyses support the potential of Cryptococcus strains as food source for P. pacificus. In summary, our work establishes two Cryptococcus strains as alternative food source for P. pacificus and shows change in various developmental, physiological and morphological traits, including the transcriptomic profiles.