Reconstruction of monocyte transcriptional regulatory network accompanies monocytic functions in human fibroblasts

• Published in: PloS one Vol. 7; no. 3; p. e33474
• Main Authors: Suzuki, Takahiro, Nakano-Ikegaya, Mika, Yabukami-Okuda, Haruka, de Hoon, Michiel, Severin, Jessica, Saga-Hatano, Satomi, Shin, Jay W, Kubosaki, Atsutaka, Simon, Christophe, Hasegawa, Yuki, Hayashizaki, Yoshihide, Suzuki, Harukazu
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 13.03.2012; Public Library of Science (PLoS)
• Subjects: Biology; Cascades; Cellular communication; Chemotaxis; Chemotaxis - immunology; Data Mining; Dendritic cells; DNA binding proteins; DNA Primers - genetics; Endocytosis; Fibroblasts; Fibroblasts - cytology; Flow Cytometry; Gene expression; Gene Expression Profiling; Gene Expression Regulation - immunology; Gene Regulatory Networks - immunology; Genes; Genetic aspects; Genetic Vectors - genetics; Genomes; Humans; Immune response; Immune system; Inflammation; Inflammatory response; Knowledge representation; Lentivirus; Lipopolysaccharides; Melanoma; Metastasis; Microarray Analysis; Monocytes; Monocytes - cytology; Monocytes - immunology; Monocytes - metabolism; Phagocytosis; Phagocytosis - immunology; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; Science; Skin; Skin - cytology; Stem cells; Transcription (Genetics); Transcription factors; Transcription Factors - immunology; Transcription Factors - metabolism; Vaccines; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0033474

Transcriptional regulatory networks (TRN) control the underlying mechanisms behind cellular functions and they are defined by a set of core transcription factors regulating cascades of peripheral genes. Here we report SPI1, CEBPA, MNDA and IRF8 as core transcription factors of monocyte TRN and demonstrate functional inductions of phagocytosis, inflammatory response and chemotaxis activities in human dermal fibroblasts. The Gene Ontology and KEGG pathway analyses also revealed notable representation of genes involved in immune response and endocytosis in fibroblasts. Moreover, monocyte TRN-inducers triggered multiple monocyte-specific genes based on the transcription factor motif response analysis and suggest that complex cellular TRNs are uniquely amenable to elicit cell-specific functions in unrelated cell types.