Effect of RECK gene polymorphisms on hepatocellular carcinoma susceptibility and clinicopathologic features

• Published in: PloS one Vol. 7; no. 3; p. e33517
• Main Authors: Chung, Tsung-Te, Yeh, Chao-Bin, Li, Yi-Ching, Su, Shih-Chi, Chien, Ming-Hsien, Yang, Shun-Fa, Hsieh, Yi-Hsien
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 12.03.2012; Public Library of Science (PLoS)
• Subjects: Alleles; Angiogenesis; Biochemistry; Biology; Cancer; Cancer genetics; Cancer metastasis; Cancer research; Carcinoma, Hepatocellular - epidemiology; Carcinoma, Hepatocellular - genetics; Case-Control Studies; Confidence intervals; Cysteine; Deoxyribonucleic acid; Disease susceptibility; DNA; DNA methylation; DNA Primers - genetics; Emergency medical care; Esophagus; Gene expression; Gene polymorphism; Genes; Genetic aspects; Genetic Association Studies; Genetic Predisposition to Disease - genetics; Genomes; Genotyping; GPI-Linked Proteins - genetics; Hepatitis; Hepatocellular carcinoma; Humans; Identification; Insulin-like growth factors; Liver cancer; Liver cirrhosis; Liver Neoplasms - epidemiology; Liver Neoplasms - genetics; Logistic Models; Lung cancer; Lymphatic system; Medical diagnosis; Medical imaging; Medical prognosis; Medicine; Metastases; Metastasis; Mortality; Neoplasm Metastasis - genetics; Patients; Polymerase chain reaction; Polymorphism, Restriction Fragment Length - genetics; Polymorphism, Single Nucleotide - genetics; Polyphenols; Promoters; Proteins; Real-Time Polymerase Chain Reaction; Restriction fragment length polymorphism; Reversion; Risk Factors; Single nucleotide polymorphisms; Single-nucleotide polymorphism; Statistics, Nonparametric; Taiwan - epidemiology; Taiwan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0033517

The reversion-inducing-cysteine-rich protein with Kazal motifs (RECK) down-regulation has been confirmed in numerous human cancers and is clinically associated with metastasis. This study investigates the potential associations of RECK single-nucleotide polymorphisms (SNPs) with hepatocellular carcinoma (HCC) susceptibility and its clinicopathologic characteristics. A total of 135 HCC cancer patients and 501 cancer-free controls were analyzed for four RECK SNPs (rs10814325, rs16932912, rs11788747, and rs10972727) using real-time PCR and PCR-RFLP genotyping analysis. After adjusting for other co-variants, the individuals carrying RECK promoter rs10814325 inheriting at least one C allele had a 1.85-fold [95% confidence interval (CI), 1.03-3.36] risk of developing HCC compared to TT wild type carriers. The HCC patients, who carried rs11788747 with at least one G allele, had a higher distant metastasis risk than wild type probands. RECK gene polymorphisms might be a risk factor increasing HCC susceptibility and distant metastasis in Taiwan.