New fluorimetric assay of horseradish peroxidase using sesamol as substrate and its application to EIA

• Published in: Journal of pharmaceutical analysis Vol. 2; no. 2; pp. 156 - 159
• Main Authors: Arakawa, Hidetoshi, Nakabayashi, Shigeo, Ohno, Ken-ichi, Maeda, Masako
• Format: Journal Article
• Language: English
• Published: China Elsevier B.V 01.04.2012; School of Pharmacy, Showa University, Shinagawa-ku, Tokyo 142-8555, Japan; Xi'an Jiaotong University; Elsevier
• Subjects: Enzyme immunoassay (EIA); Fluorescence; Horseradish peroxidase (HRP); Sesamol; Thyroid stimulating hormone (TSH); Horseradish peroxidase (HRP); Thyroid stimulating hormone (TSH); Fluorescence; Sesamol; Enzyme immunoassay (EIA)
• ISSN: 2095-1779; 2214-0883
• DOI: 10.1016/j.jpha.2012.01.004

Horseradish peroxidase (HRP) is generally used as a label enzyme in enzyme immunoassay (EIA).The procedure used for HRP detection in EIA is critical for sensitivity and precision.This paper describes a novel fluorimetric assay for horseradish peroxidase (HRP) using sesamol as substrate.The principle of the assay is as follow:sesamol (3,4-methylenedioxy phenol) is reacted enzymatically in the presence of hydrogen peroxide to produce dimeric sesamol.The dimer is fluorescent and can be detected sensitively at ...