Influence of the nanostructure of F ‐doped TiO 2 films on osteoblast growth and function

• Published in: Journal of biomedical materials research. Part A Vol. 103; no. 6; pp. 1985 - 1990
• Main Authors: Lozano, Daniel, Hernández‐López, Juan M., Esbrit, Pedro, Arenas, Maria A., Gómez‐Barrena, Enrique, de Damborenea, Juan, Esteban, Jaime, Pérez‐Jorge, Concepción, Pérez‐Tanoira, Ramón, Conde, Ana
• Format: Journal Article
• Language: English
• Published: 01.06.2015
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.35337

Abstract The aim of this study was to evaluate the proliferation and mineralization ability of mouse osteoblastic MC3T3‐E1 cells on F‐containing TiO 2 films with different morphology and nanostructure that previously confirmed antibacterial properties. F‐containing TiO 2 films were fabricated by anodizing Ti‐6Al‐4V alloy ELI ‐grade 23. By using a mixture of H 2 SO4/HF acid at 20 V for 5 and 60 min, a TiO 2 film grows with nanoporous (NP) and nanotubular (NT) features, characterized with a pore diameter of 20 and 100 nm, respectively. Fluoride‐TiO 2 barrier films (FBL) were produced in 1 M NH 4 H 2 PO 4 /0.15 M NH 4 F solution at constant voltage controlled at 20 V for 120 min. The amount of F incorporated in the nanostructured oxide films was 6 at % and of 4 at %, for the NP and NT, respectively, while for the FBL film was 12 at %. MC3T3‐E1 cells exhibited different behavior when seeded and grown onto these surfaces. Thus, F‐doped TiO 2 films with NP structures increased proliferation as well as osteogenic gene expression and the mineralization capacity of these osteoblastic cells. These results confirm that anodizing process is suitable to fabricate multifunctional surfaces on Ti‐6Al‐4V alloy with improved not only antibacterial but also osteogenic properties useful for bone fixation of prosthetic devices © 2014 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 103A: 1985–1990, 2015.