Ion-exchange Chromatography with Electrochemical Detection Using Copper Nanoparticles/Graphene Composite Film Modified Electrode for Determination of Monosaccharide

• Published in: Fēnxī huàxué Vol. 41; no. 10; pp. 1601 - 1605
• Main Authors: WANG, Feng-Li, WANG, Feng-Mei, XI, Ling-Ling, ZHU, Zuo-Yi, HUANG, Zhong-Ping, ZHU, Yan
• Format: Journal Article
• Language: English
• Published: 01.10.2013
• Subjects: CHEMICAL ANALYSIS; Chromatography; COMPOSITES; Constants; Copper; COPPER IONS; Electrodes; Graphene; MICA; MICROSTRUCTURES; Monosaccharides; Nanoparticles; Nanostructure; PARTICLES
• ISSN: 0253-3820
• DOI: 10.3724/SP.J.1096.2013.30498

A novel copper nanoparticles/graphene modified glassy carbon electrode (CuNPs-Gr/GCE) was constructed. The CuNPs-Gr nanocomposite was prepared by simultaneous electrochemical reduction using graphene oxide (GO) and copper ion as the starting materials under cyclic potential from -1.5 to 0 V. The modified electrode showed prominent electrocatalytic oxidation towards monosaccharide. Employing the CuNPs-Gr/GCE as an amperometric detector for high performance anion-exchange chromatography (HPIC), five monosaccharides (fucose, arabinose, galactose, glucose and mannose) were analyzed on-line with good reproducibility and stablity. Under constant applied potential of +0.55 V (vs Ag/AgCl), the detection limits (S/N=3) of the all investigated compounds were at the level of 0.02-0.10 mg/L. The dynamic linear ranges spanned generally three orders of magnitude with correlation coefficients more than 0.998, and the RSDs (n = 6) range from 1.9% to 2.5%. The analytical method has successfully been applied to determine the monosaccharide composition of crude polysaccharide from phellinus igniarius with acceptable level of sensitivity and recovery (from 84.8% to 94.5%). As a result, the proposed method appears to be useful and effective for the routine practical determinations of common carbohydrates in real matrices without derivatization procedures.