Phytochemistry and biological activities of the ethanolic extract of Onosma aucherianum

• Published in: Planta Medica
• Main Authors: Maškovic, P, Niciforovic, N, Solujic, S, Manojlovic, N, Cvijovic, M, Mladenovic, J, Acamovic Djokovic, G, Radojkovic, M
• Format: Conference Proceeding
• Language: English
• Published: 05.08.2011
• ISSN: 0032-0943; 1439-0221
• DOI: 10.1055/s-0031-1282889

This study was aimed at evaluating the antioxidant activity and efficacy of the ethanolic extract of the endemic plant species Onosma aucherianum DC. in inhibiting the development of selected fungi and bacteria. The highest susceptibility to the ethanolic extract of O. aucherianum among the bacteria tested was exhibited by B. subtilis and S. aureus (MIC=15.62µg/ml). Among the fungi, A. niger (MIC=15.62µg/ml) showed the highest susceptibility. Total phenolic, flavonoid, condensed tannin and gallotannin contents were 90.26±0.69mg GA/g, 35.24±0.55mg RU/g, 74.65±0.75mg GA/g and 31.74±1.05mg GA/g, respectively. Total antioxidant capacity was 78.45±0.98µg AA/g. IC 50 values were determined for each measurement: 21.45±1.55µg/ml for DPPH free radical scavenging activity, 36.46±1.68µg/ml for inhibitory activity against lipid peroxidation, 99.11±0.23µg/ml for hydroxyl radical scavenging activity and 45.91±0.88µg/ml for chelating ability. The rosmarinic acid was found to be the dominant phenolic compound of the extract. Keywords: antimicrobial activity, antioxidant activity, Onosma aucherianum, HPLC analysis, phenolic compounds Acknowledgement: Serbian Ministry of Agriculture, Forestry and Water Management, STAR Project No. 401–001972/2010–03.