Faecal Metaproteomic Analysis Reveals a Personalized and Stable Functional Microbiome and Limited Effects of a Probiotic Intervention in Adults

• Published in: PloS one Vol. 11; no. 4; p. e0153294
• Main Authors: Kolmeder, Carolin A., Salojärvi, Jarkko, Ritari, Jarmo, de Been, Mark, Raes, Jeroen, Falony, Gwen, Vieira-Silva, Sara, Kekkonen, Riina A., Corthals, Garry L., Palva, Airi, Salonen, Anne, de Vos, Willem M.
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 12.04.2016; Public Library of Science (PLoS)
• Subjects: Adult; Adults; Analysis; Bacteria; Bacterial Proteins - genetics; Bacterial Proteins - isolation & purification; Bacteriology; Biological activity; Biology; Biology and Life Sciences; Cohort Studies; Computer and Information Sciences; Disease; DNA microarrays; Double-Blind Method; Ethics; Faecalibacterium prausnitzii; Feces - microbiology; Female; Gastrointestinal Microbiome - genetics; Gene expression; Genetic aspects; Genome, Bacterial; Humans; Immunology; Intervention; Intestine; Laboratorium voor Microbiologie; Lactobacillus; Lactobacillus rhamnosus; Longitudinal Studies; Male; Medicine and Health Sciences; Metabolism; Metabolites; Microbiological Laboratory; Microbiologie; Microbiology; Microbiomes; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Middle Aged; Nutrition research; Peptide Mapping; Peptides; Phosphatase; Phylogenetics; Phylogeny; Physiological aspects; Precision Medicine; Probiotics; Probiotics - therapeutic use; Proteins; Proteomes; Proteomics; Research and Analysis Methods; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Rodents; Spectrometry; Studies; VLAG; WIMEK; Young Adult; Finland; Belgium; Netherlands
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0153294

Recent metagenomic studies have demonstrated that the overall functional potential of the intestinal microbiome is rather conserved between healthy individuals. Here we assessed the biological processes undertaken in-vivo by microbes and the host in the intestinal tract by conducting a metaproteome analysis from a total of 48 faecal samples of 16 healthy adults participating in a placebo-controlled probiotic intervention trial. Half of the subjects received placebo and the other half consumed Lactobacillus rhamnosus GG for three weeks (1010 cfu per day). Faecal samples were collected just before and at the end of the consumption phase as well as after a three-week follow-up period, and were processed for microbial composition and metaproteome analysis. A common core of shared microbial protein functions could be identified in all subjects. Furthermore, we observed marked differences in expressed proteins between subjects that resulted in the definition of a stable and personalized microbiome both at the mass-spectrometry-based proteome level and the functional level based on the KEGG pathway analysis. No significant changes in the metaproteome were attributable to the probiotic intervention. A detailed taxonomic assignment of peptides and comparison to phylogenetic microarray data made it possible to evaluate the activity of the main phyla as well as key species, including Faecalibacterium prausnitzii. Several correlations were identified between human and bacterial proteins. Proteins of the human host accounted for approximately 14% of the identified metaproteome and displayed variations both between and within individuals. The individually different human intestinal proteomes point to personalized host-microbiota interactions. Our findings indicate that analysis of the intestinal metaproteome can complement gene-based analysis and contributes to a thorough understanding of the activities of the microbiome and the relevant pathways in health and disease.