Lignans from in vitro cultures of transgenic roots of Taxus x media var. Hicksii

• Published in: Planta Medica
• Main Authors: Sykłowska-Baranek, K, Pietrosiuk, A, Grech-Baran, M, Bonfill, M, Mistrzak, P
• Format: Conference Proceeding
• Language: English
• Published: 19.07.2012
• ISSN: 0032-0943; 1439-0221
• DOI: 10.1055/s-0032-1321026

Recently, the in vitro cultures of yew transgenic roots have been demonstrated to be very potent as a source of paclitaxel and other taxanes. Lignans, dimeric phenylpropanoids, showed a great number of pharmacological effects such as antibacterial, antifungal, antiviral, antioxidant, anticancer, and anti-inflammatory. The presence of matairesinol, lariciresionol and pinoresinol has been investigated in three lines of Taxus transgenic roots. Two of them (ATMA and ATM) are carring additional taxadiene synthase gene ( txs ) while the third one – KT has been obtained after transformation with LBA 9402 Agrobacterium rhizogenes strain. The content of lariciresinol, pinoresinol and matairesinol has been examined in hairy roots cultivated in hormone free DCR-M medium or DCR-M medium supplemented with methyl jasmonate (MeJA) or with MeJA and L-phenylalanine (PHEN). The addition of MeJA to the medium resulted in the highest content of analyzed lignans. The highest yield of matairesinol (21µg/g DW), pinoresinol (17µg/g DW) and lariciresinol (1µg/g DW) has been determined in hairy roots of KT line. Among two root lines carring txs gene, line ATM accumulated higher amounts of lignans: 19µg/g DW, 13µg/g DW and 2µg/g DW, respectively.