Free Radical Scavenging Activities of Flavonoids from Cistus salviifolius L
The genus Cistus (Cistaceae) is represented by 21 species on worldwide and 5 species in Turkish flora. The Cistus genus has widespread utilization in Turkish folk medicine such as against rheumatism, for hemorrhoids, to cure sterility, kidney and urinary inflammations, as a hemostatic, antipyretic,...
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Published in | Planta Medica |
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Main Authors | , , , , |
Format | Conference Proceeding |
Language | English |
Published |
05.08.2011
|
Online Access | Get full text |
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Summary: | The genus
Cistus
(Cistaceae) is represented by 21 species on worldwide and 5 species in Turkish flora. The
Cistus
genus has widespread utilization in Turkish folk medicine such as against rheumatism, for hemorrhoids, to cure sterility, kidney and urinary inflammations, as a hemostatic, antipyretic, expectorant, sedative, and for peptic ulcer, as well as diabetes mellitus. Anti-inflammatory, anti-helicobacter pylori, antihypertensive, anti-microbial activities and cytotoxic effects of
Cistus
species were also reported. In this study, pharmacognostical investigations have been carried out on
Cistus salviifolius
L. The aerial parts of the plant were extracted with
n
-hexane and MeOH, respectively. The methanolic extract was partitioned with petroleum ether and
n
-BuOH. Isolation of secondary metabolites from
n
-BuOH extract were performed with various chromatographic methods. The
n
-BuOH extract yielded three flavonoid aglycones; CS-1: Kaempferol, CS-2: Quercetin, CS-3: Myricetin, four flavonoid glycosides; CS-4: Kaempferol 3-
O
-
β
-(6''-
O
-
trans
-
p
-coumaroyl)-galactopyranoside, CS-5: Quercetin 3-
O
-α-arabinopyranoside, CS-6: Quercetin 3-
O
-β-galactopyranoside and CS-7: Myricetin 3-
O
-
β
-galactopyranoside. The structures of these compounds were elucidated using spectroscopic methods (UV, IR,
1
H-NMR,
13
C-NMR, 2D-NMR and MS). The free radical scavenging activity of these compounds was measured by DPPH method and found as Quercetin (IC
50
: 4,23) > Kaempferol (IC
50
: 6,24) > Quercetin 3-
O
-
β
-galactopyranoside (IC
50
: 8,26) > Myricetin (IC
50
: 9,76) > Myricetin 3-
O
-
β
-galactopyranoside (IC
50
: 10,05), respectively. According to these results quercetin is more active than ascorbic acid and has similar activity with the caffeic acid used as standards. |
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ISSN: | 0032-0943 1439-0221 |
DOI: | 10.1055/s-0031-1282700 |