Adipocyte-specific protein tyrosine phosphatase 1B deletion increases lipogenesis, adipocyte cell size and is a minor regulator of glucose homeostasis

• Published in: PloS one Vol. 7; no. 2; p. e32700
• Main Authors: Owen, Carl, Czopek, Alicja, Agouni, Abdelali, Grant, Louise, Judson, Robert, Lees, Emma K, Mcilroy, George D, Göransson, Olga, Welch, Andy, Bence, Kendra K, Kahn, Barbara B, Neel, Benjamin G, Mody, Nimesh, Delibegović, Mirela
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 28.02.2012; Public Library of Science (PLoS)
• Subjects: Adipocytes; Adipocytes - cytology; Adipocytes - enzymology; Adipocytes - metabolism; Adiponectin; Adipose tissue; AKT protein; Animals; Biology; Blotting, Western; Body Composition - genetics; Body Composition - physiology; Body mass; Body weight; Brain; Cell Size; Cells, Cultured; Clinical Medicine; Clonal deletion; Endocrinology and Diabetes; Endokrinologi och diabetes; Gene deletion; Gene expression; Glucose; Glucose - metabolism; High fat diet; Homeostasis; Homeostasis - genetics; Homeostasis - physiology; Hypoxia; Immunoprecipitation; Insulin; Insulin resistance; Kinases; Klinisk medicin; Leptin; Lipid metabolism; Lipogenesis; Lipogenesis - genetics; Lipogenesis - physiology; Liver; Medical and Health Sciences; Medicin och hälsovetenskap; Medicine; Metabolism; Mice; Mice, Mutant Strains; Obesity; Phenols (Class of compounds); Phosphatases; Phosphorylation; Physiological aspects; Positron-Emission Tomography; Protein Tyrosine Phosphatase, Non-Receptor Type 1 - genetics; Protein Tyrosine Phosphatase, Non-Receptor Type 1 - metabolism; Protein-tyrosine-phosphatase; Recombinase; Rodents; Secretion; Type 2 diabetes; Tyrosine
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0032700

Protein tyrosine phosphatase 1B (PTP1B), a key negative regulator of leptin and insulin signaling, is positively correlated with adiposity and contributes to insulin resistance. Global PTP1B deletion improves diet-induced obesity and glucose homeostasis via enhanced leptin signaling in the brain and increased insulin signaling in liver and muscle. However, the role of PTP1B in adipocytes is unclear, with studies demonstrating beneficial, detrimental or no effect(s) of adipose-PTP1B-deficiency on body mass and insulin resistance. To definitively establish the role of adipocyte-PTP1B in body mass regulation and glucose homeostasis, adipocyte-specific-PTP1B knockout mice (adip-crePTP1B(-/-)) were generated using the adiponectin-promoter to drive Cre-recombinase expression. Chow-fed adip-crePTP1B(-/-) mice display enlarged adipocytes, despite having similar body weight/adiposity and glucose homeostasis compared to controls. High-fat diet (HFD)-fed adip-crePTP1B(-/-) mice display no differences in body weight/adiposity but exhibit larger adipocytes, increased circulating glucose and leptin levels, reduced leptin sensitivity and increased basal lipogenesis compared to controls. This is associated with decreased insulin receptor (IR) and Akt/PKB phosphorylation, increased lipogenic gene expression and increased hypoxia-induced factor-1-alpha (Hif-1α) expression. Adipocyte-specific PTP1B deletion does not beneficially manipulate signaling pathways regulating glucose homeostasis, lipid metabolism or adipokine secretion in adipocytes. Moreover, PTP1B does not appear to be the major negative regulator of the IR in adipocytes.