Cigarette Smoke Enhances the Expression of Profibrotic Molecules in Alveolar Epithelial Cells

• Published in: PloS one Vol. 11; no. 3; p. e0150383
• Main Authors: Checa, Marco, Hagood, James S, Velazquez-Cruz, Rafael, Ruiz, Victor, García-De-Alba, Carolina, Rangel-Escareño, Claudia, Urrea, Francisco, Becerril, Carina, Montaño, Martha, García-Trejo, Semiramis, Cisneros Lira, José, Aquino-Gálvez, Arnoldo, Pardo, Annie, Selman, Moisés
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 02.03.2016; Public Library of Science (PLoS)
• Subjects: Aberration; Activation; Alveoli; Analysis; Animals; Antigens, Neoplasm - genetics; Antigens, Neoplasm - metabolism; Biology and Life Sciences; Cell culture; Cell Line; Cigarette smoke; Cigarettes; Deregulation; DNA microarrays; Ecological risk assessment; Environmental risk; Epithelial cells; Epithelial Cells - cytology; Epithelial Cells - metabolism; Epithelial Cells - pathology; Epithelial-Mesenchymal Transition; Epithelium; Etiology; Exposure; Extracellular matrix; Fibroblasts; Fibroblasts - cytology; Fibroblasts - metabolism; Fibroblasts - pathology; Fibrosis; Gene expression; Gene Expression Regulation; Genes; Genetic aspects; Growth factors; Humans; Idiopathic Pulmonary Fibrosis - etiology; Idiopathic Pulmonary Fibrosis - genetics; Idiopathic Pulmonary Fibrosis - metabolism; Idiopathic Pulmonary Fibrosis - pathology; Insulin; Integrins - genetics; Integrins - metabolism; Laboratory animals; Lung diseases; Male; Medical research; Medicine and Health Sciences; Mice; Nicotiana - adverse effects; Pathways; Physiological aspects; Pulmonary Alveoli - cytology; Pulmonary Alveoli - metabolism; Pulmonary Alveoli - pathology; Pulmonary fibrosis; Rats, Wistar; Risk factors; Rodents; Secretion; Signal Transduction; Signaling; Smoke; Smoke - adverse effects; Smoking; Smoking - adverse effects; Transcription activation; Transcriptome; Transforming Growth Factor beta - genetics; Transforming Growth Factor beta - metabolism; Transforming growth factor-b1; Wnt protein; Wnt Signaling Pathway; Mexico
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0150383

Idiopathic pulmonary fibrosis (IPF) is a progressive and lethal disease of unknown etiology. A growing body of evidence indicates that it may result from an aberrant activation of alveolar epithelium, which induces the expansion of the fibroblast population, their differentiation to myofibroblasts and the excessive accumulation of extracellular matrix. The mechanisms that activate the alveolar epithelium are unknown, but several studies indicate that smoking is the main environmental risk factor for the development of IPF. In this study we explored the effect of cigarette smoke on the gene expression profile and signaling pathways in alveolar epithelial cells. Lung epithelial cell line from human (A549), was exposed to cigarette smoke extract (CSE) for 1, 3, and 5 weeks at 1, 5 and 10% and gene expression was evaluated by complete transcriptome microarrays. Signaling networks were analyzed with the Ingenuity Pathway Analysis software. At 5 weeks of exposure, alveolar epithelial cells acquired a fibroblast-like phenotype. At this time, gene expression profile revealed a significant increase of more than 1000 genes and deregulation of canonical signaling pathways such as TGF-β and Wnt. Several profibrotic genes involved in EMT were over-expressed, and incomplete EMT was observed in these cells, and corroborated in mouse (MLE-12) and rat (RLE-6TN) epithelial cells. The secretion of activated TGF-β1 increased in cells exposed to cigarette smoke, which decreased when the integrin alpha v gene was silenced. These findings suggest that the exposure of alveolar epithelial cells to CSE induces the expression and release of a variety of profibrotic genes, and the activation of TGF-β1, which may explain at least partially, the increased risk of developing IPF in smokers.