Oral Administration of Lactobacillus plantarum Strain AYA Enhances IgA Secretion and Provides Survival Protection against Influenza Virus Infection in Mice

• Published in: PloS one Vol. 9; no. 1; p. e86416
• Main Authors: Kikuchi, Yosuke, Kunitoh-Asari, Ayami, Hayakawa, Katsuyuki, Imai, Shinjiro, Kasuya, Kenji, Abe, Kimio, Adachi, Yu, Fukudome, Shin-ichi, Takahashi, Yoshimasa, Hachimura, Satoshi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 22.01.2014; Public Library of Science (PLoS)
• Subjects: Acids; Administration, Oral; Animals; B cells; B-Lymphocytes - immunology; B-Lymphocytes - metabolism; Bacteria; Biology; Cytokines; Cytokines - biosynthesis; Dendritic cells; Dendritic Cells - immunology; Dendritic Cells - metabolism; Female; Gene Expression; Growth factors; Health aspects; Immune system; Immunoglobulin A; Immunoglobulin A, Secretory - immunology; Immunoglobulins; Immunology; Immunoregulation; Infections; Infectious diseases; Influenza; Influenza viruses; Interleukin 6; Intestine, Small - immunology; Lactic acid; Lactic acid bacteria; Lactobacillus plantarum; Lactobacillus plantarum - immunology; Lungs; Lymphocytes B; Medicine; Mice; Mucosal immunity; Oral administration; Orthomyxoviridae - immunology; Orthomyxoviridae Infections - genetics; Orthomyxoviridae Infections - immunology; Orthomyxoviridae Infections - prevention & control; Peyer's Patches - cytology; Peyer's Patches - immunology; Plant protection; Plasma cells; Probiotics; Probiotics - administration & dosage; Quality control; R&D; Research & development; Rodents; Science; Small intestine; Survival; Viruses; Yeast; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0086416

The mucosal immune system provides the first line of defense against inhaled and ingested pathogenic microbacteria and viruses. This defense system, to a large extent, is mediated by the actions of secretory IgA. In this study, we screened 140 strains of lactic acid bacteria for induction of IgA production by murine Peyer's patch cells. We selected one strain and named it Lactobacillus plantarum AYA. We found that L. plantarum AYA-induced production of IL-6 in Peyer's patch dendritic cells, with this production promoting IgA(+) B cells to differentiate into IgA-secreting plasma cells. We also observed that oral administration of L. plantarum AYA in mice caused an increase in IgA production in the small intestine and lung. This production of IgA correlated strongly with protective ability, with the treated mice surviving longer than the control mice after lethal influenza virus infection. Our data therefore reveals a novel immunoregulatory role of the L. plantarum AYA strain which enhances mucosal IgA production and provides protection against respiratory influenza virus infection.