Grape-seed proanthocyanidin extract as suppressors of bone destruction in inflammatory autoimmune arthritis

• Published in: PloS one Vol. 7; no. 12; p. e51377
• Main Authors: Park, Jin-Sil, Park, Mi-Kyung, Oh, Hye-Joa, Woo, Yun-Ju, Lim, Mi-Ae, Lee, Jong-Ho, Ju, Ji Hyeon, Jung, Young Ok, Lee, Zang Hee, Park, Sung-Hwan, Kim, Ho-Youn, Cho, Mi-La, Min, Jun-Ki
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 10.12.2012; Public Library of Science (PLoS)
• Subjects: Acid phosphatase; Acid phosphatase (tartrate-resistant); Acid Phosphatase - metabolism; Acid resistance; Animals; Antiarthritic agents; Antioxidants; Arthritis; Arthritis, Experimental - prevention & control; Arthritis, Rheumatoid - prevention & control; Base Sequence; Biocompatibility; Biology; Biomedical materials; Bone and Bones - drug effects; Bone and Bones - pathology; Bone growth; Bone Resorption; Cartilage; Cell Differentiation - drug effects; Cells, Cultured; Collagen; Cytokines; Dentistry; Destruction; Developmental biology; Differentiation; Disease; DNA Primers; Fibroblasts; Gene expression; Grape Seed Extract - pharmacology; Homeostasis; Immunohistochemistry; In Vitro Techniques; Inflammation; Isoenzymes - metabolism; Joint diseases; Ligands; Lymphocytes; Male; Medicine; Mice; Mice, Inbred DBA; NF-κB protein; Osteoblastogenesis; Osteoblasts; Osteoblasts - drug effects; Osteoblasts - enzymology; Osteoclastogenesis; Osteoclasts - drug effects; Osteoclasts - enzymology; Peripheral blood; Phosphatases; Proanthocyanidins; Proanthocyanidins - pharmacology; Real-Time Polymerase Chain Reaction; Rheumatism; Rheumatoid arthritis; Rheumatoid factor; Science; Seeds; Suppressors; Tartrate-Resistant Acid Phosphatase; Tumor necrosis factor-TNF
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0051377

Chronic autoimmune inflammation, which is commonly observed in rheumatoid arthritis (RA), disrupts the delicate balance between bone resorption and formation causing thedestruction of the bone and joints. We undertook this study to verify the effects of natural grape-seed proanthocyanidin extract (GSPE), an antioxidant, on chronic inflammation and bone destruction. GSPE administration ameliorated the arthritic symptoms of collagen-induced arthritis (CIA), which are representative of cartilage and bone destruction. GSPE treatment reduced the formation of tartrate-resistant acid phosphatase (TRAP)-positive multinucleated cells and osteoclast activity and increased differentiation of mature osteoblasts. Receptor activator of NFκB ligand expression in fibroblasts from RA patients was abrogated with GSPE treatment. GSPE blocked human peripheral blood mononuclear cell-derived osteoclastogenesis and acted as an antioxidant. GSPE improved the arthritic manifestations of CIA mice by simultaneously suppressing osteoclast differentiation and promoting osteoblast differentiation. Our results suggest that GSPE may be beneficial for the treatment of inflammation-associated bone destruction.