Cold atmospheric plasma enhances osteoblast differentiation

This study was designed to assess the effects of cold atmospheric plasma on osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. Plasma was irradiated directly to a culture medium containing plated cells for 5 s or 10 s. Alkaline phosphatase (ALP) activity assay and alizarin red staining...

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Published inPloS one Vol. 12; no. 7; p. e0180507
Main Authors Tominami, Kanako, Kanetaka, Hiroyasu, Sasaki, Shota, Mokudai, Takayuki, Kaneko, Toshiro, Niwano, Yoshimi
Format Journal Article
LanguageEnglish
Published United States Public Library of Science 06.07.2017
Public Library of Science (PLoS)
Subjects
3T3 Cells
Alizarin
Alkaline phosphatase
Alkaline Phosphatase - genetics
Alkaline Phosphatase - metabolism
Animals
Apoptosis
Assaying
Biocompatibility
Biology and Life Sciences
Biomedical engineering
Bone growth
Bone surgery
Cell culture
Cell Differentiation
Cold
Cold effects
Cold Temperature
Dentistry
Differentiation
Electron paramagnetic resonance
Electron spin
Electron spin resonance
Electron Spin Resonance Spectroscopy
Engineering
Gases
Gene expression
Infectious diseases
Irradiation
Medicine and Health Sciences
Mice
Mineralization
Osteoblastogenesis
Osteoblasts
Osteoblasts - cytology
Osteoblasts - enzymology
Osteocalcin - genetics
Penicillin
Phosphatases
Plasma Gases
Radiation
Radicals
Regeneration
Regeneration (physiology)
Research and Analysis Methods
Resonance
Spectrum analysis
Spin resonance
Staining
Time dependence
Trapping
Ulcers
Wound healing
Japan
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Summary:This study was designed to assess the effects of cold atmospheric plasma on osteoblastic differentiation in pre-osteoblastic MC3T3-E1 cells. Plasma was irradiated directly to a culture medium containing plated cells for 5 s or 10 s. Alkaline phosphatase (ALP) activity assay and alizarin red staining were applied to assess osteoblastic differentiation. The plasma-generated radicals were detected directly using an electron spin resonance-spin trapping technique. Results show that plasma irradiation under specific conditions increased ALP activity and enhanced mineralization, and demonstrated that the yield of radicals was increased in an irradiation-time-dependent manner. Appropriate plasma irradiation stimulated the osteoblastic differentiation of the cells. This process offers the potential of promoting bone regeneration.
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content type line 23
Conceptualization: HK TK.Data curation: HK KT.Formal analysis: TM YN.Funding acquisition: HK.Investigation: HK KT SS TM.Methodology: HK TK YN.Project administration: HK.Resources: HK TK YN.Software: TM YN.Supervision: HK TK YN.Validation: HK YN.Visualization: HK KT.Writing – original draft: HK.Writing – review & editing: TK YN.
Competing Interests: The authors have declared that no competing interests exist.
These authors also contributed equally to this work.
ISSN:1932-6203
1932-6203
DOI:10.1371/journal.pone.0180507