The Cell Cycle Timing of Human Papillomavirus DNA Replication

• Published in: PloS one Vol. 10; no. 7; p. e0131675
• Main Authors: Reinson, Tormi, Henno, Liisi, Toots, Mart, Ustav, Jr, Mart, Ustav, Mart
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 01.07.2015; Public Library of Science (PLoS)
• Subjects: Amplification; Cell cycle; Cell Cycle - genetics; Copy number; Deoxyribonucleic acid; DNA; DNA biosynthesis; DNA damage; DNA polymerase; DNA Replication; DNA viruses; DNA, Viral - genetics; E2 protein; Exploitation; G2 phase; G2 Phase - genetics; Gene expression; Genetic research; Genomes; Genomics; Herpes viruses; Human papillomavirus; Humans; Papillomaviridae - genetics; Papillomavirus; Papillomavirus infections; Proteins; Replication; S phase; Synchronism; Synchronization; Synthesis; Viral Proteins - genetics; Virology; Virus Replication - genetics; Viruses
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0131675

Viruses manipulate the cell cycle of the host cell to optimize conditions for more efficient viral genome replication. One strategy utilized by DNA viruses is to replicate their genomes non-concurrently with the host genome; in this case, the viral genome is amplified outside S phase. This phenomenon has also been described for human papillomavirus (HPV) vegetative genome replication, which occurs in G2-arrested cells; however, the precise timing of viral DNA replication during initial and stable replication phases has not been studied. We developed a new method to quantitate newly synthesized DNA levels and used this method in combination with cell cycle synchronization to show that viral DNA replication is initiated during S phase and is extended to G2 during initial amplification but follows the replication pattern of cellular DNA during S phase in the stable maintenance phase. E1 and E2 protein overexpression changes the replication time from S only to both the S and G2 phases in cells that stably maintain viral episomes. These data demonstrate that the active synthesis and replication of the HPV genome are extended into the G2 phase to amplify its copy number and the duration of HPV genome replication is controlled by the level of the viral replication proteins E1 and E2. Using the G2 phase for genome amplification may be an important adaptation that allows exploitation of changing cellular conditions during cell cycle progression. We also describe a new method to quantify newly synthesized viral DNA levels and discuss its benefits for HPV research.