OASIS/CREB3L1 is induced by endoplasmic reticulum stress in human glioma cell lines and contributes to the unfolded protein response, extracellular matrix production and cell migration

• Published in: PloS one Vol. 8; no. 1; p. e54060
• Main Authors: Vellanki, Ravi N, Zhang, Liling, Volchuk, Allen
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 15.01.2013; Public Library of Science (PLoS)
• Subjects: Apoptosis; Biology; Biotechnology; Brain cancer; Brain tumors; Cell adhesion & migration; Cell Line, Tumor; Cell lines; Cell migration; Cell morphology; Cell Movement - genetics; Chondroitin sulfate; Chondroitin Sulfates - metabolism; Collagen; Collagen (type I); Cyclic AMP Response Element-Binding Protein - genetics; Cyclic AMP Response Element-Binding Protein - metabolism; Cytology; Endoplasmic reticulum; Endoplasmic Reticulum Stress - genetics; Extracellular matrix; Extracellular Matrix - metabolism; Gene expression; Gene Expression Regulation, Neoplastic; Glioma; Glioma - genetics; Glioma - metabolism; Glioma cells; Glycosylation; Health care networks; Homeostasis; Humans; Kinases; Medical research; Medicine; Molecular biology; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Physiology; Protein folding; Proteins; Proteoglycans; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; Rodents; Stress; Stress response; Stresses; Sulfate; Sulfates; Transcription factors; Tumors; Unfolded Protein Response - genetics
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0054060

OASIS is a transcription factor similar to ATF6 that is activated by endoplasmic reticulum stress. In this study we investigated the expression of OASIS in human glioma cell lines and the effect of OASIS knock-down on the ER stress response and cell migration. OASIS mRNA was detected in three distinct glioma cell lines (U373, A172 and U87) and expression levels were increased upon treatment with ER stress-inducing compounds in the U373 and U87 lines. OASIS protein, which is glycosylated on Asn-513, was detected in the U373 and U87 glioma lines at low levels in control cells and protein expression was induced by ER stress. Knock-down of OASIS in human glioma cell lines resulted in an attenuated unfolded protein response to ER stress (reduced GRP78/BiP and GRP94 induction) and decreased expression of chondroitin sulfate proteoglycan extracellular matrix proteins, but induction of the collagen gene Col1a1 was unaffected. Cells in which OASIS was knocked-down exhibited altered cell morphology and reduced cell migration. These results suggest that OASIS is important for the ER stress response and maintenance of some extracellular matrix proteins in human glioma cells.