Effects of Isoflavone-Enriched Feed on the Rumen Microbiota in Dairy Cows

• Published in: PloS one Vol. 11; no. 4; p. e0154642
• Main Authors: Kasparovska, Jitka, Pecinkova, Martina, Dadakova, Katerina, Krizova, Ludmila, Hadrova, Sylvie, Lexa, Matej, Lochman, Jan, Kasparovsky, Tomas
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 28.04.2016; Public Library of Science (PLoS)
• Subjects: Analysis; Animal Feed - analysis; Animal lactation; Animal Nutritional Physiological Phenomena - drug effects; Animals; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bacteroidetes - genetics; Bacteroidetes - isolation & purification; Betaproteobacteria - genetics; Betaproteobacteria - isolation & purification; Biochemistry; Biology and Life Sciences; Burkholderiaceae - genetics; Burkholderiaceae - isolation & purification; Cattle; Composition effects; Corn; Cow's milk; Dairy cattle; Dairy farming; Diet; Diet - veterinary; Dietary Supplements; Ecology and Environmental Sciences; Ecosystems; Enriched feed; Fatty acids; Feeds; Female; Firmicutes - genetics; Firmicutes - isolation & purification; Glycine max - metabolism; Health aspects; Isoflavones; Isoflavones - pharmacology; Lactation; Medicine and Health Sciences; Metabolism; Metabolites; Microbiomes; Microbiota; Microbiota (Symbiotic organisms); Microorganisms; Milk; Milk - chemistry; Multidimensional scaling; Planctomycetales - genetics; Planctomycetales - isolation & purification; Plant Extracts; Poaceae; Red clover; Research and Analysis Methods; RNA, Ribosomal, 16S - genetics; rRNA 16S; Rumen; Rumen - microbiology; Scaling; Science; Silage; Soy products; Statistical analysis; Studies; Trifolium pratense; Zea mays; Czech Republic
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0154642

In this study, we compared the effects of two diets containing different isoflavone concentrations on the isoflavone transfer from feed into milk and on the rumen microbiota in lactating dairy cows. The on-farm experiment was conducted on twelve lactating Czech Fleckvieh x Holstein cows divided into two groups, each with similar mean milk yield. Twice daily, cows were individually fed a diet based on maize silage, meadow hay and supplemental mixture. Control group (CTRL) received the basal diet while the experimental group (EXP) received the basal diet supplemented with 40% soybean isoflavone extract. The average daily isoflavone intake in the EXP group (16 g/day) was twice as high as that in the CTRL group (8.4 g/day, P<0.001). Total isoflavone concentrations in milk from the CTRL and EXP groups were 96.89 and 276.07 μg/L, respectively (P<0.001). Equol concentrations in milk increased from 77.78 μg/L in the CTRL group to 186.30 μg/L in the EXP group (P<0.001). The V3-4 region of bacterial 16S rRNA genes was used for metagenomic analysis of the rumen microbiome. The experimental cows exhibited fewer OTUs at a distance level of 0.03 compared to control cows (P<0.05) and reduced microbial richness compared to control cows based on the calculated Inverse Simpson and Shannon indices. Non-metric multidimensional scaling analysis showed that the major contributor to separation between the experimental and control groups were changes in the representation of bacteria belonging to the phyla Bacteroidetes, Proteobacteria, Firmicutes, and Planctomycetes. Surprisingly, a statistically significant positive correlation was found only between isoflavones and the phyla Burkholderiales (r = 0.65, P<0.05) and unclassified Betaproteobacteria (r = 0.58, P<0.05). Previous mouse and human studies of isoflavone effects on the composition of gastrointestinal microbial populations generally report similar findings.