MicroRNA-351 Regulates Two-Types of Cell Death, Necrosis and Apoptosis, Induced by 5-fluoro-2'-deoxyuridine

• Published in: PloS one Vol. 11; no. 4; p. e0153130
• Main Authors: Sato, Akira, Omi, Takuya, Yamamoto, Akihiro, Satake, Akito, Hiramoto, Akiko, Masutani, Mitsuko, Tanuma, Sei-ichi, Wataya, Yusuke, Kim, Hye-Sook
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 12.04.2016; Public Library of Science (PLoS)
• Subjects: Animals; Antineoplastic Agents - pharmacology; Apoptosis; Apoptosis - drug effects; Apoptosis - genetics; Binding sites; Biochemistry; Biology and Life Sciences; Cancer; Cancer therapies; Cell death; Cell Death - drug effects; Cell Death - genetics; Cell Line, Tumor; Chemotherapy; Cloning; Deoxyribonucleic acid; Deoxyuridine - analogs & derivatives; Deoxyuridine - pharmacology; DNA; Gangrene; Gene Expression Profiling; Genetic aspects; Genomes; Hybridization; Immunoglobulins; Informatics; Investigations; Lamin Type B - genetics; Lamin Type B - metabolism; Medicine and Health Sciences; Mice; MicroRNA; MicroRNAs; MicroRNAs - antagonists & inhibitors; MicroRNAs - genetics; MicroRNAs - metabolism; miRNA; Molecular Mimicry; Morphology; Mortality; Necrosis; Pharmaceutical sciences; Physiological aspects; Protein synthesis; Proteins; Research and Analysis Methods; Ribonucleic acid; RNA; RNA, Messenger - genetics; RNA, Messenger - metabolism; Transfection; Up-Regulation; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0153130

Cell-death can be necrosis and apoptosis. We are investigating the mechanisms regulating the cell death that occurs on treatment of mouse cancer cell-line FM3A with antitumor 5-fluoro-2'-deoxyuridine (FUdR): necrosis occurs for the original clone F28-7, and apoptosis for its variant F28-7-A. Here we report that a microRNA (miR-351) regulates the cell death pattern. The miR-351 is expressed strongly in F28-7-A but only weakly in F28-7. Induction of a higher expression of miR-351 in F28-7 by transfecting an miRNA mimic into F28-7 resulted in a change of the death mode; necrosis to apoptosis. Furthermore, transfection of an miR-351 inhibitor into F28-7-A resulted in the morphology change, apoptosis to necrosis, in this death-by-FUdR. Possible mechanism involving lamin B1 in this miR-351's regulatory action is discussed.