Inflammation-related microRNA expression level in the bovine milk is affected by mastitis

• Published in: PloS one Vol. 12; no. 5; p. e0177182
• Main Authors: Lai, Yu-Chang, Fujikawa, Takuro, Maemura, Tadashi, Ando, Takaaki, Kitahara, Go, Endo, Yasuyuki, Yamato, Osamu, Koiwa, Masateru, Kubota, Chikara, Miura, Naoki
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 17.05.2017; Public Library of Science (PLoS)
• Subjects: Acute monocytic leukemia; Adhesion; Adhesive bonding; Agriculture; Agronomy; Alveoli; Animals; Antibodies; Arteriosclerosis; Arthritis; Atherosclerosis; Attenuation; Bacteria; Bioinformatics; Biology and life sciences; Biomarkers; Blood; Blood levels; Breast cancer; California mastitis test; Cancer; Cattle; CD14 antigen; Chemical properties; Control; Dairy cattle; Degradation; Deoxyribonucleic acid; Diagnosis; Disease; Disease transmission; Diseases; DNA; E coli; Epidemiology; Female; Fluorescence; Food; Gene Expression; Gene Expression Profiling; Genomics; Glands; Health aspects; Heart diseases; Immune response; In vivo methods and tests; Infections; Infiltration; Inflammation; Inflammatory diseases; Interleukin 6; Laboratories; Leukemia; Lung cancer; Lungs; Mammary gland; Mastitis; Mastitis, Bovine - diagnosis; Mastitis, Bovine - genetics; Mastitis, Bovine - metabolism; Measurement; Medical research; Medicine; Medicine and Health Sciences; Mice; MicroRNA; MicroRNAs; MicroRNAs - genetics; Milk; Monocytic leukemia; Negative wells; Nucleotides; Parity; Pathogenesis; Probes; Proteins; Quality control; Real-Time Polymerase Chain Reaction; Research and analysis methods; Rheumatoid arthritis; Ribonucleic acid; RNA; ROC Curve; Science; Sensitivity; Signal transduction; Statistical analysis; Stimulation; Studies; Teaching hospitals; Temperature effects; Veterinary medicine; United States > US; Japan; California
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0177182

MicroRNA (miRNA) in tissue and liquid samples have been shown to be associated with many diseases including inflammation. We aimed to identify inflammation-related miRNA expression level in the bovine mastitis milk. Expression level of inflammation-related miRNA in milk from mastitis-affected and normal cows was analyzed using qPCR. We found that expression level of miR-21, miR-146a, miR-155, miR-222, and miR-383 was significantly upregulated in California mastitis test positive (CMT+) milk. We further analyzed these miRNA using a chip-based QuantStudio Digital PCR System. The digital PCR results correlated with those of qPCR, demonstrating upregulation of miR-21, miR-146a, miR-155, miR-222, and miR-383 in CMT+ milk. In conclusion, we identified miRNA that are upregulated in CMT+ milk. These miRNA exhibited sensitivity and specificity greater than 80% for differentiating between CMT+ milk and normal milk. Our findings suggest that inflammation-related miRNA expression level in the bovine milk was affected by mastitis, and miRNA in milk have potential for use as biomarkers of bovine mastitis.