Encouragement of Enzyme Reaction Utilizing Heat Generation from Ferromagnetic Particles Subjected to an AC Magnetic Field

• Published in: PloS one Vol. 10; no. 5; p. e0127673
• Main Authors: Suzuki, Masashi, Aki, Atsushi, Mizuki, Toru, Maekawa, Toru, Usami, Ron, Morimoto, Hisao
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 19.05.2015; Public Library of Science (PLoS)
• Subjects: alpha-Amylases - metabolism; Amylases; Analysis; Chitinase; Cold; Enzyme Activation; Enzymes; Ferromagnetism; Ferrous Compounds - chemistry; Fever; Heat; Heat generation; Hot Temperature; Hybrids; Hyperthermia; Interdisciplinary aspects; Magnetic fields; Magnetics; Magnetism; Microscopy, Electron, Scanning; Nanoparticles; Particulates; Polymers; Scanning electron microscopy; Science; Studies; Trends; α-Amylase
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0127673

We propose a method of activating an enzyme utilizing heat generation from ferromagnetic particles under an ac magnetic field. We immobilize α-amylase on the surface of ferromagnetic particles and analyze its activity. We find that when α-amylase/ferromagnetic particle hybrids, that is, ferromagnetic particles, on which α-amylase molecules are immobilized, are subjected to an ac magnetic field, the particles generate heat and as a result, α-amylase on the particles is heated up and activated. We next prepare a solution, in which α-amylase/ferromagnetic particle hybrids and free, nonimmobilized chitinase are dispersed, and analyze their activities. We find that when the solution is subjected to an ac magnetic field, the activity of α-amylase immobilized on the particles increases, whereas that of free chitinase hardly changes; in other words, only α-amylase immobilized on the particles is selectively activated due to heat generation from the particles.