Up-Regulation of MiR-300 Promotes Proliferation and Invasion of Osteosarcoma by Targeting BRD7

• Published in: PloS one Vol. 10; no. 5; p. e0127682
• Main Authors: Xue, Zhen, Zhao, Jindong, Niu, Liyuan, An, Gang, Guo, Yashan, Ni, Linying
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 26.05.2015; Public Library of Science (PLoS)
• Subjects: Analysis of Variance; Apoptosis; Biocompatibility; Blotting, Western; Bone cancer; Cell cycle; Cell growth; Cell Line, Tumor; Cell proliferation; Cell Proliferation - physiology; Chromosomal Proteins, Non-Histone - metabolism; Comparative analysis; Deregulation; Development and progression; Ectopic expression; Epithelial-Mesenchymal Transition - physiology; Gene expression; Gene Expression Regulation, Neoplastic - physiology; Genetic aspects; Health aspects; Humans; Luciferases; Medical research; Mesenchyme; MicroRNA; MicroRNAs; MicroRNAs - metabolism; miRNA; Neoplasm Invasiveness - physiopathology; Oligonucleotides - genetics; Osteoblasts; Osteosarcoma; Osteosarcoma - physiopathology; Physiological aspects; Physiology; Proteins; Real-Time Polymerase Chain Reaction; Sarcoma; Surgery; Tissues; Tumor suppressor genes; Variance analysis; China
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0127682

Increasing reports suggest that deregulated microRNAs (miRNAs) might provide novel therapeutic targets for cancers. However, the expression and function of miR-300 in osteosarcoma is still unknown. In our study, we found that the expression of miR-300 was up-regulated in osteosarcoma tissues and cells compared with paired adjacent non-tumor bone tissues and osteoblastic cells using RT-qPCR. The enforced expression of miR-300 could promote cell proliferation, invasion and epithelial-mesenchymal transition (EMT). Moreover, we identified that bromodomain-containing protein 7 (BRD7), a new tumor suppressor gene, was a direct target of miR-300. Ectopic expression of BRD7 could significantly inhibit miR-300-promoted proliferation, invasion and EMT. Therefore, our results identify an important role for miR-300 in osteosarcoma through regulating BRD7 expression.