Induced resistance by a long-chain bacterial volatile: elicitation of plant systemic defense by a C13 volatile produced by Paenibacillus polymyxa

• Published in: PloS one Vol. 7; no. 11; p. e48744
• Main Authors: Lee, Boyoung, Farag, Mohamed A, Park, Hyo Bee, Kloepper, Joseph W, Lee, Soo Hyun, Ryu, Choong-Min
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 28.11.2012; Public Library of Science (PLoS)
• Subjects: Acetoin; Acids; Alcohols; Arabidopsis; Arabidopsis - drug effects; Arabidopsis - growth & development; Arabidopsis - microbiology; Arabidopsis thaliana; Bacteria; Bioengineering; Biology; Butanediol; Chains; Defense industry; Disease Resistance - drug effects; Disease Resistance - genetics; Disease Resistance - immunology; Emissions; Gene expression; Gene Expression Profiling; Gene Expression Regulation, Plant; Genes; Genomes; Jasmonic acid; Laboratories; Metabolites; Mixtures; Organic compounds; Paenibacillus - metabolism; Paenibacillus polymyxa; Pathogens; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - microbiology; Plant growth; Plant protection; Pseudomonas chlororaphis; Pseudomonas syringae - immunology; Salicylic acid; Seedlings - drug effects; Seedlings - growth & development; Seedlings - microbiology; Signaling; Synthetic biology; Transcription; Transcription (Genetics); VOCs; Volatile organic compounds; Volatile Organic Compounds - chemistry; Volatile Organic Compounds - metabolism; Volatile Organic Compounds - pharmacology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0048744

Some strains of plant growth-promoting rhizobacteria (PGPR) elicit induced systemic resistance (ISR) by emission of volatile organic compounds (VOCs) including short chain alcohols, acetoin, and 2,3-butanediol. The objective of this study was to evaluate whether species-specific VOCs from PGPR strain Paenibacillus polymyxa E681 can promote growth and induce resistance in Arabidopsis. The efficacy of induction was strain-specific, with stronger protection against Pseudomonas syringae pv. maculicola ES4326 in plants exposed to VOCs from P. polymyxa E681 versus Arabidopsis plants exposed to VOCs from a reference strain Bacillus subtilis GB03, which was previously shown to elicit ISR and plant growth promotion. VOC emissions released from E681 primed transcriptional expression of the salicylic acid, jasmonic acid, and ethylene signaling marker genes PR1, ChiB, and VSP2, respectively. In addition, strain E681 produced more than thirty low molecular-weight VOCs, of which tridecane was only produced by E681 and not found in GB03 or IN937a volatile blends. These strain-specific VOCs induced PR1 and VSP2 genes. These results provide new insight into the existence of a long chain VOC signaling molecule produced by P. polymyxa that can serve as a bacterial trigger of induced systemic resistance in planta.