Suppression of respiratory growth defect of mitochondrial phosphatidylserine decarboxylase deficient mutant by overproduction of Sfh1, a Sec14 homolog, in yeast

• Published in: PloS one Vol. 14; no. 4; p. e0215009
• Main Authors: Mizuike, Aya, Kobayashi, Shingo, Rikukawa, Takashi, Ohta, Akinori, Horiuchi, Hiroyuki, Fukuda, Ryouichi
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 08.04.2019; Public Library of Science (PLoS)
• Subjects: Baking yeast; Biology and Life Sciences; Biosynthesis; Biotechnology; Blood lipids; Carbon sources; Carboxy-Lyases - deficiency; Cell Cycle Proteins - biosynthesis; Cell Cycle Proteins - genetics; Chromosomal Proteins, Non-Histone - biosynthesis; Chromosomal Proteins, Non-Histone - genetics; Collaboration; Deficient mutant; Endoplasmic reticulum; Endoplasmic Reticulum - genetics; Endoplasmic Reticulum - metabolism; Endosomes - genetics; Endosomes - metabolism; Fluorescence; Fractionation; Gene expression; Gene mutation; Genetic aspects; Genomes; Golgi apparatus; Golgi Apparatus - genetics; Golgi Apparatus - metabolism; Homology; Kinases; Lipids; Liposomes; Mammals; Membrane lipids; Membranes; Metabolism; Mitochondria; Mitochondria - genetics; Mitochondria - metabolism; Models, Biological; Oxygen Consumption; Phosphatidylethanolamine; Phosphatidylethanolamines - metabolism; Phosphatidylserine; Phosphatidylserine decarboxylase; Phosphatidylserines; Phosphatidylserines - genetics; Phosphatidylserines - metabolism; Phospholipid Transfer Proteins - genetics; Phospholipid Transfer Proteins - metabolism; Phospholipids; Plant lipids; Plasmids; Proteins; Research and Analysis Methods; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - biosynthesis; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Transport; Vacuoles - genetics; Vacuoles - metabolism; Yeast; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0215009

Interorganelle phospholipid transfer is critical for eukaryotic membrane biogenesis. In the yeast Saccharomyces cerevisiae, phosphatidylserine (PS) synthesized by PS synthase, Pss1, in the endoplasmic reticulum (ER) is decarboxylated to phosphatidylethanolamine (PE) by PS decarboxylase, Psd1, in the ER and mitochondria or by Psd2 in the endosome, Golgi, and/or vacuole, but the mechanism of interorganelle PS transport remains to be elucidated. Here we report that Sfh1, a member of Sec14 family proteins of S. cerevisiae, possesses the ability to enhance PE production by Psd2. Overexpression of SFH1 in the strain defective in Psd1 restored its growth on non-fermentable carbon sources and increased the intracellular and mitochondrial PE levels. Sfh1 was found to bind various phospholipids, including PS, in vivo. Bacterially expressed and purified Sfh1 was suggested to have the ability to transport fluorescently labeled PS between liposomes by fluorescence dequenching assay in vitro. Biochemical subcellular fractionation suggested that a fraction of Sfh1 localizes to the endosome, Golgi, and/or vacuole. We propose a model that Sfh1 promotes PE production by Psd2 by transferring phospholipids between the ER and endosome.