Green Tea Extract Rich in Epigallocatechin-3-Gallate Prevents Fatty Liver by AMPK Activation via LKB1 in Mice Fed a High-Fat Diet

• Published in: PloS one Vol. 10; no. 11; p. e0141227
• Main Authors: Santamarina, Aline B, Oliveira, Juliana L, Silva, Fernanda P, Carnier, June, Mennitti, Laís V, Santana, Aline A, de Souza, Gabriel H I, Ribeiro, Eliane B, Oller do Nascimento, Cláudia M, Lira, Fábio S, Oyama, Lila M
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 04.11.2015; Public Library of Science (PLoS)
• Subjects: Activation; Adiponectin; AMP-Activated Protein Kinases - antagonists & inhibitors; Animals; Blotting, Western; Body mass; Body Weight - drug effects; Body weight gain; Care and treatment; Catechin - analogs & derivatives; Catechin - pharmacology; Complications and side effects; Development and progression; Diabetes; Diet; Diet, High-Fat - adverse effects; Dietary supplements; Disease control; Enzyme Activation; Enzymes; Epigallocatechin gallate; Epigallocatechin-3-gallate; Esterification; Fatty acids; Fatty liver; Fatty Liver - etiology; Fatty Liver - metabolism; Fatty Liver - prevention & control; Green tea; Health aspects; High fat diet; Inflammation; Insulin resistance; Kinases; Lipoproteins (very low density); Liver; Liver diseases; LKB1 protein; Male; Metabolism; Mice; Obesity; Patient outcomes; Phosphorylation; Plant Extracts - pharmacology; Protein-Serine-Threonine Kinases - metabolism; Proteins; Regulation; Rodents; Secretion; SIRT1 protein; Sterol regulatory element-binding protein; Studies; Tea; Tea - chemistry; Weight gain measurement; United States; Brazil
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0141227

Supplementation with epigallocatechin-3-gallate has been determined to aid in the prevention of obesity. Decaffeinated green tea extract appears to restore a normal hepatic metabolic profile and attenuate high-fat diet (HFD)-induced effects, thereby preventing non-alcoholic fatty liver disease in mice. Mice were maintained on either a control diet (CD) or HFD for 16 weeks and supplemented with either water or green tea extract (50 mg/kg/day). The body mass increase, serum adiponectin level, and lipid profile were measured over the course of the treatment. Furthermore, the AMPK pathway protein expression in the liver was measured. From the fourth week, the weight gain in the CD + green tea extract (CE) group was lower than that in the CD + water (CW) group. From the eighth week, the weight gain in the HFD + water (HFW) group was found to be higher than that in the CW group. Moreover, the weight gain in the HFD + green tea extract (HFE) group was found to be lower than that in the HFW group. Carcass lipid content was found to be higher in the HFW group than that in the CW and HFE groups. Serum analysis showed reduced non-esterified fatty acid level in the CE and HFE groups as compared with their corresponding placebo groups. Increased adiponectin level was observed in the same groups. Increased VLDL-TG secretion was observed in the HFW group as compared with the CW and HFE groups. Increased protein expression of AdipoR2, SIRT1, pLKB1, and pAMPK was observed in the HFE group, which explained the reduced expression of ACC, FAS, SREBP-1, and ChREBP in this group. These results indicate that the effects of decaffeinated green tea extract may be related to the activation of AMPK via LKB1 in the liver of HFD-fed mice.