Molecular characterization of Aspergillus niger isolates inciting black mould rot of onion through RAPD

• Published in: International journal of agriculture environment & biotechnology Vol. 9; no. 4; p. 637
• Main Authors: Prajapati, B.K., Patil, R.K., Alka, Chavda, H.C.
• Format: Journal Article
• Language: English
• Published: New Delhi New Delhi Publishers 01.08.2016
• Subjects: Deoxyribonucleic acid; DNA; Genetic diversity; Genomics; Pathogens; Polymorphism; Software
• ISSN: 0974-1712; 2230-732X
• DOI: 10.5958/2230-732X.2016.00083.8

Genetic diversity in black mould rot pathogen (Aspergillus niger Van Tieghem) was analyzed using ten isolates collected from different regions of India. The genomic DNA extracted from each isolates of Aspergillus niger was subjected to polymerase chain reaction using 40 random decamer primers from OPA and OPC series. Only six primers showed amplifications and selected for the analysis. Out of these, all six primers showed 100 per cent polymorphism. The total number of amplified fragments was 159, with a range of 10 to 38 fragments per primer. Dendrogram generated by pooled molecular data of six RAPD primers formed two clusters namely 'A' and 'B'. The cluster 'A' was divided into AN-1 and AN-4 isolates, Cluster B was divided into two sub-clusters B^sub 1^ and B^sub 2^. Sub-cluster B^sub 1^ included B^sub 11^ and B^sub 12^ cluster. Cluster B^sub 11^ included isolates viz., AN-02, AN-03, AN-05 and AN-06. Cluster B^sub 12^ included isolate AN-09. Sub-cluster B^sub 2^ included B^sub 21^ and B^sub 22^ cluster. B^sub 21^ cluster included isolate AN-07 and B^sub 22^ cluster included isolates AN-08 and AN-10. The similarity coefficient ranged from 0.037 to 0.56 with all the six primers. Highest similarity (0.56) was observed between AN-5 and AN-6 isolates, while lowest similarity (0.037) was observed between AN-1 and AN-2 isolates. Thus, the molecular characterization of ten isolates of A. niger by RAPD revealed existence of variations.