Stochastic switching induced adaptation in a starved Escherichia coli population

• Published in: PloS one Vol. 6; no. 9; p. e23953
• Main Authors: Shimizu, Yoshihiro, Tsuru, Saburo, Ito, Yoichiro, Ying, Bei-Wen, Yomo, Tetsuya
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 13.09.2011; Public Library of Science (PLoS)
• Subjects: Adaptation; Adaptation, Biological - genetics; Analysis; Bacteria; Biology; Biosynthesis; Bistability; Cell growth; Chemical reactions; Circuits; Data analysis; E coli; Enzymes; Escherichia coli; Escherichia coli - cytology; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli - physiology; Gene expression; Gene Expression Regulation, Bacterial; Genes; Genes, Bacterial - genetics; Information science; Lactose; Lactose - metabolism; Nutritional status; Physiological aspects; Population; Population number; Promoter Regions, Genetic - genetics; Science; Signal transduction; Stochastic Processes; Stochasticity; Stress, Physiological - genetics; Switching; Transient receptor potential proteins; Tryptophan; Tryptophan - deficiency; Japan
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0023953

Population adaptation can be determined by stochastic switching in living cells. To examine how stochastic switching contributes to the fate decision for a population under severe stress, we constructed an Escherichia coli strain crucially dependent on the expression of a rewired gene. The gene essential for tryptophan biosynthesis, trpC, was removed from the native regulatory unit, the Trp operon, and placed under the extraneous control of the lactose utilisation network. Bistability of the network provided the cells two discrete phenotypes: the induced and suppressed level of trpC. The two phenotypes permitted the cells to grow or not, respectively, under conditions of tryptophan depletion. We found that stochastic switching between the two states allowed the initially suppressed cells to form a new population with induced trpC in response to tryptophan starvation. However, the frequency of the transition from suppressed to induced state dropped off dramatically in the starved population, in comparison to that in the nourished population. This reduced switching rate was compensated by increasing the initial population size, which probably provided the cell population more chances to wait for the rarely appearing fit cells from the unfit cells. Taken together, adaptation of a starved bacterial population because of stochasticity in the gene rewired from the ancient regulon was experimentally confirmed, and the nutritional status and the population size played a great role in stochastic adaptation.