Lunar phase-dependent expression of cryptochrome and a photoperiodic mechanism for lunar phase-recognition in a reef fish, goldlined spinefoot

• Published in: PloS one Vol. 6; no. 12; p. e28643
• Main Authors: Fukushiro, Masato, Takeuchi, Takahiro, Takeuchi, Yuki, Hur, Sung-Pyo, Sugama, Nozomi, Takemura, Akihiro, Kubo, Yoko, Okano, Keiko, Okano, Toshiyuki
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 07.12.2011; Public Library of Science (PLoS)
• Subjects: Animals; Biology; Birds; Brain; Brain - metabolism; Circadian Rhythm; Circadian rhythms; Cloning, Molecular; Coral reefs; CRY protein; Cryptochromes - chemistry; Cryptochromes - metabolism; Diencephalon; DNA Primers - genetics; Drosophila; Electrical engineering; Female; Fish; Fishes; Fishes - physiology; Gene expression; Genes; Insects; Light; Lunar rhythm; Medicine; Mesencephalon; Messenger RNA; Moon; Night; Oncorhynchus mykiss; Ovary - metabolism; Photoperiod; Photoreception; Photoreceptors; Phylogenetics; Phylogeny; Physiological aspects; Recognition; Reef fish; RNA, Messenger - metabolism; Science; Sparus aurata; Spawning; Spawning behavior; State variable; Japan; Okinawa
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0028643

Lunar cycle-associated physiology has been found in a wide variety of organisms. Recent study has revealed that mRNA levels of Cryptochrome (Cry), one of the circadian clock genes, were significantly higher on a full moon night than on a new moon night in coral, implying the involvement of a photoreception system in the lunar-synchronized spawning. To better establish the generalities surrounding such a mechanism and explore the underlying molecular mechanism, we focused on the relationship between lunar phase, Cry gene expression, and the spawning behavior in a lunar-synchronized spawner, the goldlined spinefoot (Siganus guttatus), and we identified two kinds of Cry genes in this animal. Their mRNA levels showed lunar cycle-dependent expression in the medial part of the brain (mesencephalon and diencephalon) peaking at the first quarter moon. Since this lunar phase coincided with the reproductive phase of the goldlined spinefoot, Cry gene expression was considered a state variable in the lunar phase recognition system. Based on the expression profiles of SgCrys together with the moonlight's pattern of timing and duration during its nightly lunar cycle, we have further speculated on a model of lunar phase recognition for reproductive control in the goldlined spinefoot, which integrates both moonlight and circadian signals in a manner similar to photoperiodic response.