Hopanoid-free Methylobacterium extorquens DM4 overproduces carotenoids and has widespread growth impairment

• Published in: PloS one Vol. 12; no. 3; p. e0173323
• Main Authors: Bradley, Alexander S, Swanson, Paige K, Muller, Emilie E L, Bringel, Françoise, Caroll, Sean M, Pearson, Ann, Vuilleumier, Stéphane, Marx, Christopher J
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 20.03.2017; Public Library of Science (PLoS)
• Subjects: Antibiotics; Bacteria; Bacteriology; Biochemistry, Molecular Biology; Biology and Life Sciences; Carotenoids; Carotenoids - biosynthesis; Carotenoids - metabolism; Cell Membrane - metabolism; Cellulase; Cellulase - metabolism; Cellulose; Culture Media; Enzymes; Evolutionary biology; Fatty acids; Fatty Acids - metabolism; Flocculation; Fluidity; Geobiology; Geochemistry; Gram-negative bacteria; Growth; Growth conditions; Growth disorders; Hydrogen-Ion Concentration; Life Sciences; Lipid A; Lipids; Localization; Membrane Fluidity; Membrane Lipids - physiology; Metabolism; Methylobacterium extorquens; Methylobacterium extorquens - genetics; Methylobacterium extorquens - growth & development; Methylobacterium extorquens - metabolism; Methylobacterium extorquens - physiology; Microbiology and Parasitology; Molecular biology; Mutants; Mutation; Osmolar Concentration; Osmotic stress; Permeability; pH effects; Physical Sciences; Physiological aspects; Physiology; Rhodopseudomonas palustris; Secretion; Sediments; Sterols; Strain; Stress, Physiological; Temperature; Toxins
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0173323

Hopanoids are sterol-like membrane lipids widely used as geochemical proxies for bacteria. Currently, the physiological role of hopanoids is not well understood, and this represents one of the major limitations in interpreting the significance of their presence in ancient or contemporary sediments. Previous analyses of mutants lacking hopanoids in a range of bacteria have revealed a range of phenotypes under normal growth conditions, but with most having at least an increased sensitivity to toxins and osmotic stress. We employed hopanoid-free strains of Methylobacterium extorquens DM4, uncovering severe growth defects relative to the wild-type under many tested conditions, including normal growth conditions without additional stressors. Mutants overproduce carotenoids-the other major isoprenoid product of this strain-and show an altered fatty acid profile, pronounced flocculation in liquid media, and lower growth yields than for the wild-type strain. The flocculation phenotype can be mitigated by addition of cellulase to the medium, suggesting a link between the function of hopanoids and the secretion of cellulose in M. extorquens DM4. On solid media, colonies of the hopanoid-free mutant strain were smaller than wild-type, and were more sensitive to osmotic or pH stress, as well as to a variety of toxins. The results for M. extorquens DM4 are consistent with the hypothesis that hopanoids are important for membrane fluidity and lipid packing, but also indicate that the specific physiological processes that require hopanoids vary across bacterial lineages. Our work provides further support to emerging observations that the role of hopanoids in membrane robustness and barrier function may be important across lineages, possibly mediated through an interaction with lipid A in the outer membrane.